Chem 104 Lab Manual Fall 2016 - Chemistry 104 Laboratory...

Chemistry 104 Laboratory Manual
The City College of New York
FALL 2016

Contents
Chemistry 104 Laboratory Syllabus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . i
Chemistry 104 Laboratory Safety Agreement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Experiment 1 Colligative Properties: Freezing-Point Depression and Molar Mass . . . . . . . 1
Experiment 2 Computer Analysis of Reaction Kinetics Data . . . . . . . . . . . . . . . . . . . . 13
Experiment 3 Kinetics of the Reaction of FD&C Red #3 with Bleach . . . . . . . . . . . . . . . 17
Experiment 4 Enzyme Catalysis with Peroxidase . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Experiment 5 Chemical Equilibrium: Formation Constant of a Complex Ion System . . . . . 37
Experiment 6 Hydrogen Phosphate Buffer System . . . . . . . . . . . . . . . . . . . . . . . . . 49
Experiment 7 Analysis of Antacids by Back Titration . . . . . . . . . . . . . . . . . . . . . . . . 61
Experiment 8 Buffer Capacity of the NH
3
/NH
4
Cl Buffer System . . . . . . . . . . . . . . . . . . 69
Experiment 9 Electrochemistry: Silver Ion Equilibrium . . . . . . . . . . . . . . . . . . . . . . 79
Appendix I General Laboratory Techniques . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
Appendix II Spectronic 20D+ Spectrophotometer . . . . . . . . . . . . . . . . . . . . . . . . 99
Appendix III Hanna Instruments pH meter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101

Chemistry 104 Laboratory Syllabus
Laboratory Schedule :
Lab # Date
Topic
1 8/25–8/31
Check in
Introduction to lab policies and safety rules
2 8/29, 9/1–9/7 Colligative Properties: Freezing-Point Depression and Molar Mass
3 9/8–9/14 Computer Analysis of Reaction Kinetics Data
4 9/15–9/21 Kinetics of the Reaction of FD&C Red #3 with Bleach
5 9/22–9/28 Enzyme Catalysis with Peroxidase
6
9/29–9/30,
10/5–10/6,
10/14
Chemical Equilibrium: Formation Constant of a Complex Ion System, Part A
— 10/7, 10/13 NO LAB (Applies ONLY to Chem 104 lab)
7 10/17–10/21 Chemical Equilibrium: Formation Constant of a Complex Ion System, Part B
8 10/24–10/28 Hydrogen Phosphate Buffer System pre-lab work
9 10/31–11/4 Hydrogen Phosphate Buffer System
10 11/7–11/11 Analysis of Antacids by Back Titration
11 11/14–11/18 Buffer Capacity of the NH
3
/NH
4
Cl Buffer System
— 11/21–11/23 NO LAB (Applies ONLY to Chem 104 lab)
12 11/28–12/2 Electrochemistry: Silver Ion Equilibrium, Part A
13 12/5–12/9
Electrochemistry: Silver Ion Equilibrium, Parts B & C
Check out
Please note that there are no labs scheduled on the following dates: 10/7, 10/13, 11/21, 11/22, 11/23.
i

Grading: Lab reports are due at the end of each lab period and will be graded based on your experimental
results, data treatment, and answers to post-lab questions. Part of the grade for each report will include a
score for overall lab performance. This score will be based on your preparedness, attendance, adherence
to safety rules, experimental technique, and work ethic. You will incur point penalties if you arrive late or
unprepared, fail to abide by the safety rules or follow directions, and for being uncooperative or disruptive
to the class. For most experiments, you will be required to complete a pre-lab assignment. Pre-lab
assignments are located at the end of the corresponding section in the lab manual and are due at the
beginning of the lab period. A detailed point breakdown is given below.
Colligative Properties: Freezing-Point Depression and Molar Mass . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 points
Computer Analysis of Reaction Kinetics Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 points
Kinetics of the Reaction of FD&C Red #3 with Bleach . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 points
Enzyme Catalysis with Peroxidase . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 points
Chemical Equilibrium: Formation Constant of a Complex Ion System (2 weeks) . . . . . . . . . . . . . . . . 40 points