lab micro#3 - Title and Number: Aseptic Techniques and...

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Title and Number:Aseptic Techniques and Culturing Microbes Lab#2Purpose: This laboratory main purpose was to aquaierd good aseptic techniques and to createcultures of bacteria and yeast for this and for future experiments.Procedure:The first step was to gather all the instruments necessary for the experiment . Afterwashing our hands ,the next step is to sanitize the workarea with 10% bleach solution, putsafety gloves, face mask, apron, and goggle, than mix the nutrient broth with E.coli tablet bymixing .25 ml of the nutrient broth in the E.coli tablet, shake until its blended and with apipet,transfer the mixture to the container with nutrient broth, using the aseptic techniques weproced to seal the nutrient broth containing the E.coli cultureand incubate for 48 hours. Weuse the same procedure for S.epidermidis, and S.cerevisiae. With the exception that forS.cerevisiae culture we mixed ½t easpoonn of the yeast packet with ¼ cup of warm water.After the 48 hours of incubation, we appliedd the same Aseptique techniquess to prepare ourwork area. We gathered 3 agar plates and label with the name of the sample that will be spread.We used a sterile loop to transfer a small sample of the brothto the agar plate and spread it

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Term
Spring
Professor
humphrey
Tags
Microbiology, Bacteria, Agar plate, Nutrient Broth

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