FAQ1

FAQ1 - About DNA synthesis DNA are commonly prepared...

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DNA are commonly prepared synthesized (i.e. prepared) by one of the three different methods 1. Chemical synthesis (usually <100nt): short ssDNA can be chemically synthesized by a "oligonulceotide synthesizer" machine. You can also synthesize the two complementary strands to make a dsDNA. 2. Enzymatic synthesis (usually <10 kp): dsDNA can be synthesized in vitro (in test tube), it needs a primer, a template (DNA or RNA), a DNA polymerase , e.g. PCR, RT (reverse transcription), nick translation, random priming. 3. Biological synthesis (can be kb to Mb): ssDNA and dsDNA can be synthesized in vivo (usually in bacterial cells), such as plasmid, phages, etc. It needs a vector and a host cell line. About DNA synthesis
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For a probe to work, it needs to be: 1. As long as possible to the limit of chemical DNA synthesis 2. As specific as possible, i.e. as low degeneracy (total number of possible sequences) as possible Codon vs degeneracy
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DNA polymerase vs Klenow fragment Klenow fragment is part of the DNA polymerase, which synthesizes
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This note was uploaded on 02/20/2009 for the course LS 3 taught by Professor Lin during the Spring '06 term at UCLA.

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FAQ1 - About DNA synthesis DNA are commonly prepared...

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