In class notes for December 12, 2007

In class notes for December 12, 2007 - bloodstream? • Use...

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Introduction to Biological Sciences In class notes for December 12, 2007 Combinatorial-(for any given gene, you have a different combination of modules based on the expression of the gene) and modular-(various modules are used to regulate genes, they are composed of binding sites in the extended promoter of a certain gene and whether or not there are certain transcription factors bound there) Gene on slide o Expressed (transcribed) only in pancreas cells In an early embryo you have a low level of expression, because it is constituent (Always active) R2- Is a negative element, C+ is the positive element. In an adult you have a moderate level of expression Under certain conditions, you need even more expression. This condition is in the presence of a certain hormone in the blood stream. You have an active protein bound to the C element Next slide o Deletion analysis of an extended promoter How is the insulin gene indicated in relation to the glucose in the
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Unformatted text preview: bloodstream? • Use PCR to amplify the promoter • Ligate to a reporter gene (in a plasmid or a vector) o A reporter gene has two characteristics that are important in this: It encodes a protein that is easy to detect. The protein is normally not found in either the cells or the organism that you are studying. o Examples Beta galactosidase (lac Z gene) Introduction to Biological Sciences In class notes for December 12, 2007 Green flourescent protein (turns green with a certain wavelength of light on it, it does not kill the cells like you have to with beta galactosidase. • Delete regions of promoter • See if there is an effect on the production of reporter protein. Lac Z is hooked to a particular promoter, it has a core promoter and an extended promoter. We look at expression under two conditions....
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This note was uploaded on 02/22/2009 for the course BSCI 110A taught by Professor Broadie/patton during the Fall '08 term at Vanderbilt.

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In class notes for December 12, 2007 - bloodstream? • Use...

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