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Unformatted text preview: bloodstream? • Use PCR to amplify the promoter • Ligate to a reporter gene (in a plasmid or a vector) o A reporter gene has two characteristics that are important in this: It encodes a protein that is easy to detect. The protein is normally not found in either the cells or the organism that you are studying. o Examples Beta galactosidase (lac Z gene) Introduction to Biological Sciences In class notes for December 12, 2007 Green flourescent protein (turns green with a certain wavelength of light on it, it does not kill the cells like you have to with beta galactosidase. • Delete regions of promoter • See if there is an effect on the production of reporter protein. Lac Z is hooked to a particular promoter, it has a core promoter and an extended promoter. We look at expression under two conditions....
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This note was uploaded on 02/22/2009 for the course BSCI 110A taught by Professor Broadie/patton during the Fall '08 term at Vanderbilt.
- Fall '08