This preview shows pages 1–3. Sign up to view the full content.
This preview has intentionally blurred sections. Sign up to view the full version.View Full Document
Unformatted text preview: Introduction to Biological Sciences 110a In class notes for November 12, 2007 Gene expression Transcription Bacteria Core polymerase o Alpha, beta, beta prime, w o Elongation o Termination Holopolymerase o Alpha, beta, beta prime, w and o o Initiation o Sigma subunit o Provides promoter specificity There are several different sigma subunits o Mostly 70 promotes with consensus sequences presented last time o Most cells only have 70 however under certain conditions you lose this one and gain 32 . This happens when you have heat shock. o This alters the sequence the holopolymerase binds to o Turning this on and off is a way of global regulation of large sets of genes Weve talked about promoters, composition of polymerase molecule, and termination SEE A Binding is more stable than nonbinding Some of the binding energy is used to unwind and unpair a region of DNA of about 20 bp SEE B NTPs diffuse in, SEE C Int roduction to Biological Sciences 110a In class notes for November 12, 2007...
View Full Document