In class notes for October 31, 2007

In class notes for October 31, 2007 - end of the nick o The...

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Introduction to Biological Sciences 110a In class notes for October 31, 2007 Replication Orgins and Initiation Eukaryotes - - - - - ---- ----- ----- ----- ----- ------- -------- -------- ------- ----- ------------------------------------------------ Bacteria/ Archaea 1 Origin that is bidirectional We can zoom into only one side
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Introduction to Biological Sciences 110a In class notes for October 31, 2007 The strand that is 3’ 5’ relative to the direction of the fork movement is the leading strand template Replication fork machinery o Polymerase III, , and δ ε o Primase (RNA polymerase) o H-helicase o Topoisomerae o SSB (single stranded binding proteins) One SSB binds to 5- 15 or 20 base pairs When an orgin is coverging
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Introduction to Biological Sciences 110a In class notes for October 31, 2007 Filling in a nick o Polymerase I (B) binds to a nick o It uses the 5’ to 3’ exonucleae activity to remove 1 nucleotide from the 3’
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Unformatted text preview: end of the nick o The result is a gap of one nucleotide and generates a 1 base template. A gap is missing one or more nucleotides. This would be something like an A missing a T or a G missing a C. o Uses polymerization activity to add a new dNTP to the one that was just removed o See A o We still have a nick We broke a bond and made a new one, replacing the nucleotide. Introduction to Biological Sciences 110a In class notes for October 31, 2007 This process repeats over and over again for about 30 to 50 cycles and then it tends to just fall off. So at this point the RNA primer is gone o The enzyme used to get rid of the nick is called ligase. This is not a favorable reaction. It uses ATPAMP + Pi and an enzyme Ligase also requires a nick with the sugars being deoxy A 3OH A single 5 phosphate on the other side of the nick...
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This note was uploaded on 02/22/2009 for the course BSCI 110A taught by Professor Broadie/patton during the Fall '08 term at Vanderbilt.

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In class notes for October 31, 2007 - end of the nick o The...

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