bis_104_quiz_3_key_ss_i_08

bis_104_quiz_3_key_ss_i_08 - BIS 104 SS I, 2008 Name _...

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BIS 104 Name ____________________________________ SS I, 2008 (last) (first) QUIZ #3 The diagram below depicts a differentiated arterial endothelial cell showing the distribution and relative amounts of two membrane-associated proteins. Protein A is an external peripheral protein with a relative mobility on SDS-PAGE of 0.75 (R m = 0.75). Protein B is an integral membrane protein with an external domain and R m = 0.40. Question #1 on the next page refers to this cell and its two membrane proteins, A & B.
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This signature affirms that I have complied with the U C Davis Honor Code. Signature Name ____________________________________ 1. Plasma membranes were isolated from a pure population of these cells and the membrane proteins were analyzed by SDS-PAGE. Using the three SDS-PAGE lanes given below, sketch in and label the results you would expect to observe from the following experiments: (6 pts) a) lane #1: sample = a total protein extract from the plasma membrane of untreated cells; gel stained with Coomassie Blue
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b) lane #2: sample = a total protein extract from cells previously exposed to trypsin; gel stained with
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bis_104_quiz_3_key_ss_i_08 - BIS 104 SS I, 2008 Name _...

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