bis_104_ss_i_final_exam_key_08

bis_104_ss_i_final_exam_key_08 - Page 1 Name PART I 1 Fill...

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Page 1 Name ______________________________ PART I 1. Fill in the blank with the term or phrase that most precisely fits the description. (14 pts – no partial credit) a. A video-enhanced microscopic technique used to monitor the movements of an individual molecule in the plane of a membrane: Single Particle Tracking (SPT) b. A technique used to measure the rate of lateral mobility of a membrane lipid or protein: Fluorescence Recovery After Photobleaching (FRAP) c. Because of this optical phenomenon, light emanating from a point in the specimen can never be seen as a point in the image, but only as a small disk: Diffraction d. The ability to see two neighboring points in the visual field as two distinct entities: Resolution (resolving power) e. The major structural protein encasing a coated pit: Clathrin f. Transport of a vesicle toward the periphery of a cell: Anterograde transport g. The enzyme responsible for removing the signal sequence from proteins in the RER: Signal peptidase
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h. Carrier proteins that transport two solutes simultaneously in opposite directions: Antiporter i. The type of facilitated transport protein that can be regulated by voltage-gating: Channel protein j. The sub-cellular site of steroid and lipid synthesis: Smooth Endoplasmic Reticulum (SER) k. The process by which LDLs are internalized by cells: Receptor Mediated Endocytosis (RME) l. Family of motor proteins that move cargo in the plus (+) direction along microtubules: Kinesins m. Organelle responsible for degrading defective or aged proteins. Proteasome n. Cell type that synthesizes insulin. Beta cell ( B -cell; pancreatric B -cell) Page 2 Name ______________________________ 2. Describe the results you would expect from the following experiments (20 pts) a. Undifferentiated (PECAM-1 pos. / ephrinB2 neg.) ECs from mouse limb skin were grown in culture and exposed to 2.5 pM VEGF. After a 2-day exposure, ECS were tested for ephrinB2 mRNA expression and compared to control ECs that were not exposed to VEGF.
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Treated cells: ~ 50% of the cells showed an increase in expression of ephrinB2 mRNA. Control cells: showed little or no increase in ephrinB2 mRNA expression . b. Peripheral sensory nerves (PSNs) were separated into populations of Schwann cells and neurons by FACS. The neurons were then tested for the expression of glial fibrillar acidic protein (GFAP) by RT-PCR followed by northern blotting. No detectable expression of GFAP specific mRNA was found in the PCR products from neuron cytoplasmic mRNA. c.
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This note was uploaded on 02/23/2009 for the course BIS 104 taught by Professor Scholey during the Spring '08 term at UC Davis.

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bis_104_ss_i_final_exam_key_08 - Page 1 Name PART I 1 Fill...

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