bis_104_mid_i_exam_key_08

bis_104_mid_i_exam_key_08 - BIS 104-002 Spring, 2008 Name_...

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BIS 104-002 Name________________________________ Spring, 2008 (last) (first) Exam #: ______ MIDTERM EXAMINATION I Cover sheet: do not detach INSTRUCTIONS: There are 5 pages not counting the cover sheet. Print your name on each page. Write clearly in the spaces provided; illegible answers will receive a score of zero. Point values are given in parentheses; 60 points total My signature affirms that I have complied with the U C Davis Honor Code. signature Page 2 Name ______________________________
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5. What information does the technique of freeze-fracture replication provide regarding bio-membrane (e.g., plasma membrane) composition? (2) Reveals the presence or absence of integral (transmembrane) proteins; the abundance, relative size, and their distribution pattern within the membrane . 6. What microscopic technique is used to view membrane specimens prepared by freeze-fracture replication? (2) Electron microscopy. (specifically, scanning) (SEM) 7. What two structural features of membrane lipids influence the relative fluidity of a bio-membrane? (2) a ) phospholipid fatty acid chain length: longer chains = less fluidity (greater viscosity ) b ) Degree of saturation of phospholipid fatty acid chains: less saturation (more double bonds) = greater fluidity. 8. What influence does cholesterol have on the transition temperature of a bio-membrane? (2) Cholesterol acts as a “buffer” to broaden the transition range so a membrane can remain functional over a broader range of temps. (In general, increased cholesterol decreases membrane permeability and fluidity). Page 1 Name ______________________________
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1. In your research lab you have a microscope with the following specifications: An ocular lens (eyepiece) capable of 10x magnification. An objective lens (oil immersion)
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bis_104_mid_i_exam_key_08 - BIS 104-002 Spring, 2008 Name_...

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