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extra credit - Marcus Knowlton In the exploration of the...

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Marcus Knowlton In the exploration of the antifungal activity of parotid secretion, an attempt was made to find out which negatively charged proteins in the secretions of parotid secretion could or could not be responsible for fungal resistance in the mouth. To do this they placed a Lashley cup in two patients’ mouth to extract secretions and then treated it by boiling the secretions, conducting a cation exchange through resin, which removed all of the positively charged proteins. The reason that the positively charged proteins were removed was because we have already identified which positively charged proteins were responsible for antifungal properties of the secretions. After the secretions were run through the resin they were separated via electrophoresis and then taken and put into a fungal growth broth along with fungal spores from the fungus Candida Kefyr. After the results were analyzed it was found that there were some negatively charged proteins in the parotid secretions that were responsible for antifungal properties. After this the
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extra credit - Marcus Knowlton In the exploration of the...

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