ZieglerLecture11-Glycolysis-PyruvateFates-ppt1

ZieglerLecture11-Glycolysis-PyruvateFates-ppt1 - Lecture...

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Lecture 11: Lecture 11: Glycolysis Glycolysis and the and the Fates of Fates of Pyruvate Pyruvate BIOCHEMISTRY 100 Winter 2009 M. Ziegler
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Learning Objectives Learning Objectives 1. Structures: see “vocabulary” list (not repeated here). 2. Terminology (explain/defne; understand use oF terms): “investment” phase, “pay-oFF” phase, substrate level phosphorylation 3. Write all 10 reactions oF glycolysis in order (or any one oF them), with structures oF substrates and products, and names oF enzymes, and describe what each reaction is “accomplishing”. 4. State in which steps in glycolysis ATP is invested and in which steps ATP is produced. 5. Explain (thermodynamics/Free energy coupling) why a compound with a phosphate group whose Free energy oF hydrolysis is more negative can transFer phosphate to another compound to make a phosphorylated product whose Free energy oF hydrolysis is less negative (e.g., ATP + glucose -->ADP + glucose-6-P; or PEP + ADP --> pyruvate + ATP) 6. What are the products oF the frst phase oF glycolysis (reactions 1-5)? Is there any oxidation oF glucose in the frst 5 reactions? Why is the 5th reaction (triose phosphate isomerase reaction) needed? 7. What are the starting compounds (and stoichiometry with respect to each glucose that entered glycolysis) and overall products oF the second phase oF glycolysis (reactions 6-10). Which reaction(s) involve oxidation? reduction oF NAD + to NADH? substrate level phosphorylation?
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Learning Objectives, continued 8. At what step in glycolysis is the substrate committed to continue in the glycolytic pathway? 9. What is fructose- 2 ,6-bisphosphate -- where does it come from, how is its production regulated, and why/how is its concentration important in regulation of the rate of glycolysis? (Relate this to hormonal control of glycolysis via the hormones glucagon and insulin.) 10. How many electrons are transferred in a redox reaction in which an aldehyde is oxidized to a carboxylic acid? 11. How is some of the free energy from the exergonic oxidation of glyceraldehyde-3-phosphate conserved in the product molecule for future use (in next reaction)? What kind of phosphate linkage is in 1,3- bisphosphoglycerate that permits it to phosphorylate ADP? 12. What kind of phosphate linkage is in PEP that permits it to phosphorylate ADP? 13. What are the 3 catabolic fates of pyruvate? Write the anaerobic reactions (substrates, products, names of enzyme) for lactate fermentation and for conversion of pyruvate to alcohol (2 reactions). What is the most important product of these 2 sets of reactions, when [O 2 ] is limiting? 14. Write the overall reaction for conversion of pyruvate to acetyl-CoA, the name of the enzyme complex, and the list of 5 coenzymes it uses.
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Glycolysis Glycolysis (the (the Glycolytic Glycolytic Pathway) Pathway) Figure 17.1 Glucose is converted to 2 pyruvate One 12 Oz. Can One 12 Oz. Can Of Coke has 39g of sucrose. There are sucrose. There are 342.3g of sucrose per mole. Sucrose per mole. Sucrose is a disaccharide composed of composed of glucose and fructose. How fructose. How much ATP can you make from this?
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This note was uploaded on 02/25/2009 for the course BCH 100 taught by Professor Staff during the Spring '08 term at UC Riverside.

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ZieglerLecture11-Glycolysis-PyruvateFates-ppt1 - Lecture...

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