23_Lieb_euk_gene_reg

23_Lieb_euk_gene_reg - Biology 202- Lieb Eukaryotic Gene...

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Biology 202- Lieb Eukaryotic Gene Regulation Page 1 of 2 Introduction There are major differences between prokaryotic and eukaryotic transcriptional regulation. You should know what these are. I. Cis -acting sequences core promoter : This refers to the area very near transcription start site, includes TATA box promoter-proximal elements : 100-200 bp from start, includes CAAT box or GC-rich sequence enhancers (for positive control) and silencers (for negative control) can act at large distances from the transcription start site, even downstream. The typically function even if they are moved or inverted, and will work to control expression of heterologous promoters. A typical gene is regulated by complex combinations of enhancers and silencers. Enhancers are thought to function by looping to interact with the core promoter. II. Trans -acting factors (transcription factors) Basal transcription factors (TFIIA, TFIIB, etc.) bind to the core promoter to assist RNA polymerase. TFIID includes TBP (TATA-binding protein) and TAFs (TBP-associated factors); TFIIH includes some DNA repair proteins. Transcriptional activators
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This note was uploaded on 03/02/2009 for the course BIOL 201 taught by Professor Mitchell during the Spring '07 term at UNC.

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23_Lieb_euk_gene_reg - Biology 202- Lieb Eukaryotic Gene...

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