26_Lieb_RNA processing

26_Lieb_RNA processing - Biology 202- Lieb RNA Processing...

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Biology 202- Lieb RNA Processing Page 1 of 2 I. RNA processing in eukaryotes Transcription takes place in the nucleus, but translation occurs in the cytoplasm. RNAs undergo several processing steps before being sent to the cytoplasm. The RNA synthesized by RNA polymerase is called the primary transcript or pre-mRNA . After all processing is done, it is called an mRNA . capping : 7-methylguanosine is added to the 5' end, through a 5' to 5' triphosphate linkage. polyadenylation : A stretch of A's (up to a few hundred) is added to the 3' end of the mRNA. Addition occurs after cleaving the mRNA just after a polyadenylation signal with the consensus sequence AAUAAA ). The polyadenylation tract is non-templated – it does not exist in the DNA sequence. The polyadenylation tract is useful experimentally, since it provides a way to separate mRNA from other RNAs. II. Splicing Eukaryotic genes are often "interrupted" by sequences that get removed to make the mRNA. The parts that removed are called
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26_Lieb_RNA processing - Biology 202- Lieb RNA Processing...

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