Lab Memo 3 - Carey Beth Condrey 10/02/2008 Lab 3 Thin Layer...

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Carey Beth Condrey 10/02/2008 Lab 3 – Thin Layer Chromatography Post Lab Objectives The purpose of this experiment was to observe the effects of polarity on interactions between a solvent, the solid phase, and a sample compound by performing thin layer chromatography. Our knowledge of polarity and molecular interactions was then applied in the creation of a solvent that would produce maximum separation between four different organic compounds. Observations The three plates in Part 1 dealt with the pigments found in spinach leaves. Each plate produced a different separation, with different pigments appearing. The pigments were only qualitatively classified in this lab memo, as spectroscopy was not available. The mixed solution of 75:25 hexane:ethyl acetate produced the best results, and the spots were clearly distinguishable from each other. The 100% ethyl acetate solution, however, produced a single yellow spot and a long, lime green tail. Part 2 of this experiment involved the creation of various solvents in order to produce a plate with an optimum separation of 0.35 (R F value) for the triphenylmethanol. The first solvent tested produced a R F value of 0.6875; while this is not very close to the optimum separation value, all subsequent solvent
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This note was uploaded on 03/02/2009 for the course CHEM 2545 taught by Professor Csantos during the Fall '08 term at Virginia Tech.

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Lab Memo 3 - Carey Beth Condrey 10/02/2008 Lab 3 Thin Layer...

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