enzyme lab

enzyme lab - THE EFFECT OF TEMPERATURE, PH, AND SUBSTRATE...

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THE EFFECT OF TEMPERATURE, PH, AND SUBSTRATE INHIBITORS OF VARYING CONCENTRATIONS ON THE RATE OF ENZYME- CATALYZED REACTIONS USING ALKALINE PHOSPHATASE ABSTRACT The effect of the enzyme alkaline phosphatase was assayed at pH levels ranging from 4.0 to 12.0 and experimented with temperatures ranging from 0 o C to 100 o C. The results found that optimal pH for this particular enzyme was 10.0 and absorbed best at a temperature of 100 o C. The enzyme was also studied using the substrate inhibitors molybdate, inorganic phosphate, and phenyl phosphonateon the substrate pNPP. The results found that inorganic phosphate proved to be the best inhibitor and phenyl phosphonate to be the worst inhibitor. INTRODUCTION Enzymes are easily one of the most important biological molecules that are crucial to the everyday functioning of living organisms. As such understanding factors such as pH, temperature, and substrate inhibitors are crucial when looking at individual enzymes in order to determine the optimal conditions for activity. The enzyme specifically studied was alkaline phosphatase which is crucial in removing phosphate groups from biological molecules such as proteins, nucleotides, and alkaloids. In this specific experiment molybdate, inorganic phosphate, and phenyl phosphonate, was used to represent the substrate inhibitors blocking the enzyme from
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This note was uploaded on 03/02/2009 for the course BIO 101 taught by Professor Martin during the Fall '08 term at Rutgers.

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enzyme lab - THE EFFECT OF TEMPERATURE, PH, AND SUBSTRATE...

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