%2332+-+Light+Microscopy - BME 418 Quantitative Cell...

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BME 418, Quantitative Cell Biology Alan J. Hunt #32: Light Microscopy Perhaps the single most important technique for cell biology is microscopy. Although almost everyone is familiar with simple microscopy, modern techniques go far beyond the microscopes that most of us have used in grade school to look at pond water. This survey will introduce the most important techniques. This is by no means exhaustive, and since the optics go well beyond the scope of this course, we won’t do justice to the physics. The intent is to become familiar with commonly used techniques, and the advantages/disadvantages of each. Mitosis, 1882 (Walther Flemming) Mitosis, 2003 (Conly Rieder) The basic principal of magnification with a lens: 1/ p + 1/ q = 1/ f Magnification = p / q An important improvement was the invention of the compound microscope. In this design a second lens (eye piece) is introduced to further magnify the virtual image from the first lens (objective). A later improvement was the invention of “infinity corrected optics”. With this design, imaging is less sensitive on the optical pathlength between the objective and the tube lens. This makes imaging more robust to perturbations caused when auxiliary optics are placed behind the objective lens.
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BME 418, Quantitative Cell Biology Alan J. Hunt “Infinity Corrected” microscope optics.
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