05EnzymesInhibitionModePotencyDetermine

05EnzymesInhibitionModePotencyDetermine - Single Substrate...

Info iconThis preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
1 Single Substrate Michaelis- Menten Kinetics S ubstrate P roduct E nzyme [E] : Brackets means concentration of that thing 2 What happens when you add reversible enzyme inhibitors? Reversible means no permanent change to enzyme; typically, non-covalent interaction We will initially study a mode of reversible inhibition Called competitive inhibition . There are other modes of inhibition too.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
3 Competitive Inhibition The inhibitor competes with the substrate for the active site of the enzyme. E + S ES E + P k 1 k -1 k 2 4 Models of Competitive Inhibition Often competitive inhibitors resemble the substrate so that it specifically binds to the active site but cannot react.
Background image of page 2
5 Enzyme Inhibitors Approximately one-half of the top drugs sold worldwide are enzyme inhibitors. Inhibitors can block certain metabolic processes. Decrease concentrations of products Increase concentrations of substrates Many diseases arise from either an excess of a product or a deficiency of a substrate, and the use of enzyme inhibitors can be extremely beneficial in treating these disorders. Useful in examining enzyme mechanisms. Can provide information about the active site of the enzyme. Aid in understanding the physiological or pathological role of the enzyme pathway.
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 13

05EnzymesInhibitionModePotencyDetermine - Single Substrate...

This preview shows document pages 1 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online