08MultiEnzymeSystems-EGFsignaling

08MultiEnzymeSystems-EGFsignaling - Basic cell building...

Info icon This preview shows pages 1–8. Sign up to view the full content.

View Full Document Right Arrow Icon
Basic cell building block + Signal transduction Gumbiner, B., Cell adhesion:The molecular basis of tissue archtecture and morphogenesis, Cell. 84, 345-357 (1996) Materials effects Medium effects
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
How Does Receptor Density Affect Subcellular Signaling and Behavior? Sawano, Takayama, Matsuda, Miyawaki Dev. Cell 2002 Local EGF Delivery Local Signaling? Global Signaling? Subcellular Stimulation and Cancer Cancer cells: uncoordinated, moves a lot Cancer cells: often overexpress growth factor receptors (EGF) Iressa: small molecule kinase inhibitor Herceptin: antibody to EGF receptor
Image of page 2
Types of papers Review Paper Tischer & Bastiaen Nat. Rev. Mol. Cell. 2003 , 4 , 971-974. Original Research Reynolds, Tischer et al. Nat Cell Biol 2003 , 5, 447-453. Ras Ras signaling signaling Ras is a G-protein GDP bound inactive GTP bound active GEF (guanine nucleotide exchange factor) activates GAP (GTPase activating protein) inactivates Ras Ras Raf MAPK/ERK Signal Signal Genes Genes Ras inactive Ras active GEF GAP
Image of page 3

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Raichu Raichu - Ras Ras How to sense active Ras? Raf binds active Ras RBD: Ras binding domain of Raf YFP, CFP
Image of page 4
Treatment of Parts of Single Cells Using Laminar Flow Localized EGF Signals in Normal Cell Cell Movemen Sawano, Takayama, Matsuda, Miyawaki Dev. Cell 2002
Image of page 5

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
What is this movie telling you? What is this movie telling you? About Ras Activation, and why? About EGFR activation, and why? What is the right hand overlay telling you, and why? Ligand Ligand- independent Lateral Propagation independent Lateral Propagation of EGF Signals in a Cancer of EGF Signals in a Cancer- like Cell like Cell Sawano, Takayama, Matsuda, Miyawaki Dev. Cell 2002
Image of page 6
Local versus Global Activation of
Image of page 7

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 8
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern