09AlteringCellularInformation

09AlteringCellularInformation - Altering Cellular...

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Altering Cellular Information Chapter 2, Chapter 8, Chapter 14. Sometimes happens naturally Cancer Can also be engineered by design The FRET imaging experiments Basics Gene Expression Basics Transcription, Translation, Splicing Prokaryote vs Eukaryote Cloning of plasmid DNA Restriction enzymes Ligase Polymerase chain reaction (PCR) Transformation of competent cells Transfection of mammalian cells Transient vs Stable transfection
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When a protein is needed by a cell, the genetic code for that protein must must be read from the DNA and processed. Enzymes carry out these processes. A two step process: 1. Transcription = synthesis of a single-stranded RNA molecule using the DNA template (1 strand of DNA is transcribed). 2. Translation = conversion of a messenger RNA sequence into the amino acid sequence of a polypeptide (i.e., protein synthesis) Gene Expression Gene Expression
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Eukaryotic Cells Have Eukaryotic Cells Have Introns Introns
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mRNA Differences between prokaryotes and eukaryotes: Prokaryotes 1. mRNA transcript is mature, and used directly for translation
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This note was uploaded on 03/03/2009 for the course BIOMEDE 321 taught by Professor Takayama during the Winter '08 term at University of Michigan.

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09AlteringCellularInformation - Altering Cellular...

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