09AlteringCellularInformation - Altering Cellular...

Info icon This preview shows pages 1–5. Sign up to view the full content.

Altering Cellular Information Chapter 2, Chapter 8, Chapter 14. Sometimes happens naturally Cancer Can also be engineered by design The FRET imaging experiments Basics Gene Expression Basics Transcription, Translation, Splicing Prokaryote vs Eukaryote Cloning of plasmid DNA Restriction enzymes Ligase Polymerase chain reaction (PCR) Transformation of competent cells Transfection of mammalian cells Transient vs Stable transfection
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

When a protein is needed by a cell, the genetic code for that protein must must be read from the DNA and processed. Enzymes carry out these processes. A two step process: 1. Transcription = synthesis of a single-stranded RNA molecule using the DNA template (1 strand of DNA is transcribed). 2. Translation = conversion of a messenger RNA sequence into the amino acid sequence of a polypeptide (i.e., protein synthesis) Gene Expression Gene Expression
Image of page 2
Eukaryotic Cells Have Eukaryotic Cells Have Introns Introns
Image of page 3

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

mRNA Differences between prokaryotes and eukaryotes: Prokaryotes 1.
Image of page 4
Image of page 5
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern