10HowCellsGrow - Single Cell Type; Batch Reactor Substrate...

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1 Single Cell Type; Batch Reactor S ubstrate + Cells P roduct + More Cells X : cell mass S ubstrate P roduct E nzyme N : cell number
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2 Batch Cell Growth Cell growth is an autocatalytic reaction substrates + cells products + more cells Rate of growth (mass) Net specific growth rate: Rate of growth (cell number) Net specific replication rate: Gross specific growth rate – Loss of cell mass How Cells Grow How Cells Grow 12 3 4 5 - Log Scale, Exponential P. 161
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3 Phases of Batch Growth Cycle Lag Phase Adaptation of cells to new environment Nutrients, growth factors Age In Enzyme Reaction, initial lag phase was very short 1 Phases of Batch Growth Cycle Exponential Phase Balanced growth Growth rate independent of nutrient concentration Enzyme Reaction Was linear 2
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4 Balanced growth Doubling time of cell mass = Doubling time of cell number Growth rate depends only on amount of cells there are that can divide (not nutrient) Doubling Time Phases of Batch Growth Cycle Declining Growth Phase Nutrient depletion Unbalanced growth (Cell size composition changes) 3 Enzyme Reaction Was Similar
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5 Phases of Batch Growth Cycle Stationary Phase
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10HowCellsGrow - Single Cell Type; Batch Reactor Substrate...

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