11GrowthKinetics - Single Cell Type Batch Reactor Substrate...

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Single Cell Type; Batch Reactor S ubstrate + Cells P roduct + More Cells X : cell mass S ubstrate P roduct E nzyme N : cell number
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How Cells Grow How Cells Grow 1 2 3 4 5 - Log Scale, Exponential - Particles vs Optical Density vs Viable cells P. 161 Exponential Phase Balanced growth Growth rate independent of nutrient concentration 2 V
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Substrate Limited Cell Growth Substrate Limited Cell Growth Enzyme Reaction E. coli growth on glucose (glucose is limiting substrate) How would you model this growth kinetics? Monod Model Based on E. coli growth on glucose Assumes a single growth limiting substrate Largely empirical (premise that single enzyme system with M-M kinetics that is responsible for S uptake is rate limiting growth) µ m : Max specific growth rate K s : Saturation constant or Half-velocity constant µ net = µ g if endogeneous metabolism is unimportant
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Other models Monod equation good for slow growth, low density culture.
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