13Oxygen-BioreactorDesign

13Oxygen-BioreactorDesign - Single Cell Type; Batch Reactor...

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Single Cell Type; Batch Reactor S ubstrate + Cells P roduct + More Cells X : cell mass S ubstrate P roduct E nzyme N : cell number Substrate Limited Cell Growth Substrate Limited Cell Growth E. coli growth on glucose (glucose is limiting substrate) How would you model this growth kinetics?
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Comparison of conc. driving forces and uptake rates for glucose and oxygen by yeast Problems encountered in oxygen transport can be illustrated by comparing transport of glucose vs oxygen; 1% Sugar (glucose) Broth O 2 sat @ 25 o C Conc. in bulk broth 10,000 ppm approx. 7 ppm Critical conc 100 ppm 0.7 ppm Rate of demand 2.8 mmoles/g cells/h 7.7 mmoles/g cells/h Need to constantly supply! Critical dissolved oxygen levels for a range of microorganisms Organism Temperature Critical dissolved o C Oxygen concentration (mmoles dm -3 ) Azotobacter sp. 30 0.018 E. coli 37 0.008 Saccharomyces sp. 30 0.004 Penicillium chrysogenum 24 0.022 Azotobacter vinelandii is a large, obligately aerobic soil bacterium which has one of the highest respiratory rates known among living organisms
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Tissue Oxygen Concentration Percentage (%) Ambient air 21 Artery 13 Vein 3.2 Bone marrow 5 Brain 3-4 Epidermis 3-5 Kidney 1.7-2.1 Liver 4.34 Muscle 2.7-4 Oviduct 5 Spleen 3.1 Testis 1.7 Air 21% Alveoli 14% Artery 13% Vein 3% Testis 1.7% Muscle 2.7-4% Oviduct 5% Bone Marrow 5% Brain 3-4% Mean Oxygen Concentration Mean Oxygen Concentration in Various Tissues in Various Tissues Important for regulation Of function (growth, differentiation, death, Secretion, etc) RESPIRATION RATE (Demand) The effect of dissolved oxygen on the specific uptake rate (i.e respiration or growth) is described by Michaelis Menton or Monod type relationship Respiration rate ( q O2 )= q max . O 2 conc / ( Ks + O 2 conc) (mg O2/g DCW hour) or µ = µ max . C/ (Ks + C) where C = oxygen conc.
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This note was uploaded on 03/03/2009 for the course BIOMEDE 321 taught by Professor Takayama during the Winter '08 term at University of Michigan.

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13Oxygen-BioreactorDesign - Single Cell Type; Batch Reactor...

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