13Oxygen-BioreactorDesign - Single Cell Type Batch Reactor...

Info icon This preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
Single Cell Type; Batch Reactor S ubstrate + Cells P roduct + More Cells X : cell mass S ubstrate P roduct E nzyme N : cell number Substrate Limited Cell Growth Substrate Limited Cell Growth E. coli growth on glucose (glucose is limiting substrate) How would you model this growth kinetics?
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Comparison of conc. driving forces and uptake rates for glucose and oxygen by yeast Problems encountered in oxygen transport can be illustrated by comparing transport of glucose vs oxygen; 1% Sugar (glucose) Broth O 2 sat @ 25 o C Conc. in bulk broth 10,000 ppm approx. 7 ppm Critical conc 100 ppm 0.7 ppm Rate of demand 2.8 mmoles/g cells/h 7.7 mmoles/g cells/h Need to constantly supply! Critical dissolved oxygen levels for a range of microorganisms Organism Temperature Critical dissolved o C Oxygen concentration (mmoles dm -3 ) Azotobacter sp. 30 0.018 E. coli 37 0.008 Saccharomyces sp. 30 0.004 Penicillium chrysogenum 24 0.022 Azotobacter vinelandii is a large, obligately aerobic soil bacterium which has one of the highest respiratory rates known among living organisms
Image of page 2
Tissue Oxygen Concentration Percentage (%) Ambient air 21 Artery 13 Vein 3.2 Bone marrow 5 Brain 3-4 Epidermis 3-5 Kidney 1.7-2.1 Liver 4.34 Muscle 2.7-4 Oviduct 5 Spleen 3.1 Testis 1.7 Air 21% Alveoli 14% Artery 13% Vein 3% Testis 1.7% Muscle 2.7-4% Oviduct 5% Bone Marrow 5% Brain 3-4% Mean Oxygen Concentration Mean Oxygen Concentration in Various Tissues in Various Tissues Important for regulation Of function (growth, differentiation, death, Secretion, etc) RESPIRATION RATE (Demand) The effect of dissolved oxygen on the specific uptake rate (i.e respiration or growth) is described by Michaelis Menton or Monod type relationship Respiration rate ( q O2 ) = q O2 max . O 2 conc / ( Ks + O 2 conc) (mg O2/g DCW hour) or µ = µ max . C/ (Ks + C) where C = oxygen conc.
Image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern