16MaterialsBAlanceStoichiometry-2008

16MaterialsBAlanceStoichiometry-2008 - • Electrons that...

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1 Stoichiometry of Growth and Product Formation Just like with cars you want high gas mileage, you want economic media for cells. More cells/products for your buck. Common parameters useful to estimate Y or other parmeters Yield based on ATP or electron or oxygen • Table 6.1 oxygen Y
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2 Predict yield/need as easily as possible What is easiest to measure • Example 7.2 page 215.
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3 •T ab l e 7 .1 ATP Y Advantage of Y X/ATP etc Regularities: some parameters same regardless of S used. Useful for estimating Y M
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4 • Example 7.2 page 215.
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5 Yield Based on Available Electrons Take into account energetics of reaction How much energy available in substrate
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Unformatted text preview: • Electrons that would be transferred to oxygen upon oxidation of a compound to CO 2 , H 2 O, NH 3 . • C = 4; H = 1, N = -3, O = -2; P = 5; S = 6. • Aerobic growth Yx/e- = 3.14 +/- 0.11 gm DCW/ e- (when NH3 is N source) 6 Theoretical Estimation of Cellular Yield, Y x/s (yield coefficient) Available Electron Method” (Wang et al, 1979) Aerobic growth Yx/e-= 3.14 +/- 0.11 gm DCW/ e-(when NH 3 is N source) e.g. C 6 H 12 O 6 + 6 O 2----> 6 CO 2 + 6 H 2 O the available electrons in 1 mole of glucose is 6(4) + 12(1) + 6(-2) = 24 electrons; based on O 2 it is 6(4) Thus, Y x/s on glucose = [Yx/e-(24 electrons)]/180 = (3.14) (24)/180 = 75.36/180 = 0.42 gm DCW/gm glucose...
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This note was uploaded on 03/03/2009 for the course BIOMEDE 321 taught by Professor Takayama during the Winter '08 term at University of Michigan.

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16MaterialsBAlanceStoichiometry-2008 - • Electrons that...

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