17ComplexBioreactor-2008

17ComplexBioreactor-2008 - Single Cell Type; Continuous...

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1 Single Cell Type; Continuous Culture S ubstrate + Cells P roduct + More Cells X : cell mass S ubstrate P roduct E nzyme N : cell number Somewhat Complex Bioreactors Chemostat with recycle Stability Higher Cell Conc Multi-stage chemostat Stability Non-growth associated product Fed-batch reactors Maintain growth phase/subst conc like continuous No wash out
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2 Chemostat with Cell Recycle Chemostat with Cell Recycle From Monod Eq S doesn’t depend on S 0
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3 Chemostat with Cell Recycle 1: without recycle 2: with recycle; alpha = 0.5; C = 2.0 μ m = 1 h -1 ; S 0 =2.0 g/L; Ks = 0.010 g/L; Yx/s = 0.5 g/g Chemostat with Cell Recycle Because cell growth is autocatalytic, more cells produces more cells; X is bigger. Can consume more substrate/time Can use D greater than μ m . Minimizes process perturbation (waste water treatment, feed stream quality may change)
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4 Multi-Stage Chemostat Chemostat frozen in a particular phase of growth Separation of growth and product-formation Genetic instability of genetically engineered cells: Growth without induction….doesn’t get diluted out by wild type (WT) Induction causes engineered cells to use much energy
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This note was uploaded on 03/03/2009 for the course BIOMEDE 321 taught by Professor Takayama during the Winter '08 term at University of Michigan.

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17ComplexBioreactor-2008 - Single Cell Type; Continuous...

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