17degreeReductionComplexBioreactor

17degreeReductionComplexBioreactor - Stoichiometry of...

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Stoichiometry of Growth and Product Formation Just like with cars you want high gas mileage, you want economic media for cells. More cells/products for your buck. Common parameters useful to estimate Y or other parmeters Yield based on ATP or electron or oxygen Predict yield/need as easily as possible What is easiest to measure
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Example 7.2 page 215. Yield Based on Available Electrons Take into account energetics of reaction How much energy available in substrate Electrons that would be transferred to oxygen upon oxidation of a compound to CO 2 , H 2 O, NH 3 . C = 4; H = 1, N = -3, O = -2; P = 5; S = 6. Aerobic growth Yx/e- = 3.14 +/- 0.11 gm DCW/ e- (when NH3 is N source)
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Theoretical Estimation of Cellular Yield, Y x/s (yield coefficient) Available Electron Method” (Wang et al, 1979) Aerobic growth Yx/e - = 3.14 +/- 0.11 gm DCW/ e - (when NH 3 is N source) e.g. C 6 H 12 O 6 + 6 O 2 ----> 6 CO 2 + 6 H 2 O the available electrons in 1 mole of glucose is 6 x 4 = 24 electrons Thus, Y x/s on glucose = [Yx/e - (24 electrons)]/180 = (3.14) (24)/180 = 75.36/180 = 0.42 gm DCW/gm glucose
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Single Cell Type; Continuous Culture S ubstrate + Cells P roduct + More Cells X : cell mass S ubstrate P roduct E nzyme N : cell number
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  • Winter '08
  • Takayama
  • Enzyme, Technol Biol Effective, Substrate Enzyme Substrate, Biol Effective visuals, visuals Extentive Res, Res earch QA

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