18Sterilization-2008 - Sterilization Why sterilize The...

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1 Sterilization Why sterilize The sterilization bioreaction Effect of scaling on sterilization Methods Why Sterilize? Sterility – NO living organisms Economic penalty is high for loss of sterility Contamination Vaccines must have only killed viruses Recombinant DNA fermentations - exit streams must be sterilized
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2 Sterilization Agents Thermal - preferred for economic large-scale sterilizations of liquids and equipment Chemical - preferred for heat-sensitive equipment ethylene oxide (gas) for equipment 70% ethanol-water (pH 2) for equipment/surfaces 3% sodium hypochlorite for equipment Radiation - UV for surfaces, x-rays for liquids (cost/safety) Filtration membrane filters have uniform micropores depth filters of glass wool Thermal Sterilization Heat susceptibility is organism dependent. Decimal reduction time (D Time) - Time to kill 90 % or 1 log unit of a population at a given temperature. Moist heat - Acts by denaturation and coagulation of proteins. To kill vegetative bacteria, yeast and mold -- 80º C for 5 -10 minutes; Mold spores -- 80º C for 30 minutes; Bacterial spores -- 121º C, 15 lbs/sq. in. pressure for at least 15 minutes.
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