CH 9 Outline - DNA The Molecule of Heredity 0 Chapter 9 0...

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Chapter 9 0 DNA: The Molecule of Heredity
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Miescher Discovered DNA 1868 Johann Miescher investigated the chemical composition of the nucleus Isolated an organic acid that was high in phosporus He called it “nuclein” We call it DNA (deoxyribonucleic acid) today 0
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Mystery of the Hereditary Material Originally believed to be an unknown class of proteins Rationale was… Heritable traits are diverse Molecules encoding traits must be diverse Proteins are made of 20 amino acids and are structurally diverse 0
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Structure of the Hereditary Material Experiments in the 1950s showed that DNA is the hereditarty material Scientists raced to determine the structure of DNA 1953- Watson and Crick proposed that DNA is a double helix 0
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Discovery of DNA Funtion: 3 critical experiments 0
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Early and puzzling clues 0
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1928 Attempting to develop a vaccine Isolated two strains of Streptococcus pnemoniae Rough strain (R) was harmless Smooth strain (S) was pathogenic 0 #1: Griffith Discovers Transformation”
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Griffith Discovers Transformation 1. Mice injected with live cells of harmless strain R. 2. Mice injected with live cells of killer strain S. 3. Mice injected with heat-killed S cells. 4. Mice injected with live R cells plus heat-killed S cells. R S dead S dead S R + dead S dead S X X 0
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Transformation What happened in the fourth experiment? The harmless R cells had been transformed by material from the dead S cells Descendents of the transformed cells were also pathogenic 0
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0 Living R strain Living S strain Heat-killed S strain Living R strain, heat-killed S strain Bacterial strain(s) injected into mouse Results Conclusions R strain does not cause pneumonia.
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