CH 13_student_outline

CH 13_student_outline - 0 Biotechnology Chapter 13...

Info iconThis preview shows pages 1–12. Sign up to view the full content.

View Full Document Right Arrow Icon
Biotechnology Chapter 13 0
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Biotechnology is applied biology Modern focus on genetic engineering, recombinant DNA technology, and analysis of biomolecules Traditional (historical) applications of biotechnology date back to over 10,000 years ago Use of yeast to produce beer and wine in Egypt and Near East Selective breeding of plants and animals Biotechnology
Background image of page 2
Genetic Engineering 0 Genetic engineering refers to the modification of genetic material to achieve specific goals Major goals of genetic engineering Learn more about cellular processes, including inheritance and gene expression Provides better understanding and treatment of diseases, particularly genetic disorders Generate economic and social benefits through production of valuable biomolecules and improved plants and animals for agriculture
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Genes are isolated, modified, and inserted into an organism Made possible by recombinant technology Cut DNA up and recombine pieces Amplify modified pieces Various techniques used to accomplish this Modified animals are called transgenic or genetically modified organisms (GMOs) Recombinant DNA
Background image of page 4
Discovery of Restriction Enzymes Hamilton Smith was studying how Haemophilus influenzae defend themselves from bacteriophage attack Discovered bacteria have an enzyme that chops up viral DNA 0
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Specificity of Cuts R estriction enzymes cut DNA at a specific sequence Number of cuts made in DNA will depend on number of times the “target” sequence occurs For example: 5’-ATT ѵ AAT-3’ The “diamond” symbol indicates where the cut is made Recognize “palindromic” sequences 0
Background image of page 6
5’-CGGGCGCATTAATCCCATTAATGCG-3’ 5’-CGGGCGC ATTAAT CCC ATTAAT GCG-3’ 5’-CGGGCGC ATT AAT CCC ATT AAT GCG-3’ Cut DNA with Eco RI restriction enzyme 0 In linear DNA, 2 restriction sites present will cut the DNA into 3 fragments
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Making Recombinant DNA 5’ 3’ G C T T A A A A T T C G G A A T T C C T T A A G 3’ 5’ one DNA fragment another DNA fragment 3’ 5’ 0
Background image of page 8
Making Recombinant DNA nick 5’ 3’ 3’ 5’ G A A T T C C T T A A G nick G A A T T C C T T A A G DNA ligase action 0
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Using Plasmids Plasmid is small circle of bacterial DNA Foreign DNA can be inserted into plasmid Forms recombinant plasmids Plasmid is a cloning vector Can deliver DNA into another cell 0
Background image of page 10
Using Plasmids DNA fragments + enzymes recombinant plasmids host cells containing recombinant plasmids 0
Background image of page 11

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 12
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 03/03/2009 for the course BIOL 1001 taught by Professor Minor during the Spring '08 term at LSU.

Page1 / 45

CH 13_student_outline - 0 Biotechnology Chapter 13...

This preview shows document pages 1 - 12. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online