lab 8 biofluorescence detection answers

lab 8 biofluorescence detection answers - M287L Lab Report...

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M287L Lab Report 8 ~ Part IV Homework Questions: 1. Can you design a fluorescent experiment to detect an antibody by using an antigen that will bound to the antibody? Yes. The principle is to fluorescence molecules to bind with antigen, and then mix the antibody with antigen to detect if there is any fluorescence in the microscope. So firstly, we need find a kind of agent like BSA, which is used to immobilize the sample on the substrate after special treatment. Likewise, it should be able to bind to antigens. Secondly, I will immobilize these molecules with antigens at the other end with certain concentration onto a proper substrate. Thirdly, I will add certain fluorescent molecules into the 'solution to be detected. Finally, I will put one droplet of the treated solution on the substrate and let them re~ct for a while. Then I will check the fluorescence intensity under the jluorescence microscope. / If the fluorescence intensity is very high, it means that there are des. d antibodies in the solution, vice versa.
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