Fluctuation test - Luria-Delbrck Fluctuation Test tonB...

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1 Luria-Delbrück Fluctuation Test tonB Positive selection
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2 Inoculate flask with bacteria Then Either continue growth Or Divide into smaller flasks Newcombe’s Experiment
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3 Joshua & Esther Lederberg - Replica plating Adaptive Mutation Adaptive Mutation Thoughts from 2000 A stress-related or stress-induced phenomenon E. coli FC40 Δ lac Chromosome Plasmid F’lac - +1 frameshift Mutation rate during non-selective growth: 1 in 10 9 cells per generation Mutation rate during selective growth: 1 in 10 9 cells per hour But they also contain many more non-selected mutations! Almost exclusively -1 deletions Recombination functions required Only observed if lac is episomal “Selective” mutants are different
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4 Almost exclusively -1 deletions Suggests that DNA synthesis is occurring Recombination functions required Recombination does require some degree of replication and DNA synthesis Is this synthesis error-prone?
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  • Fall '08
  • SOOWAL
  • Genetics, Bacteria, DNA, Mutation, adaptive mutation, Joshua D. Tompkins, frameshift Δlac, spontaneous duplication Recombination, duplication Recombination drives

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