in_vivo_microscopy - Looking deep within The exciting...

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Looking deep within: The exciting prospect of in vivo microscopy. Introduction: Medical science is progressing at a tremendous rate and and the trend that is currently generating a lot of interest is personalized diagnosis, treatment and disease
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management. Decisions that are specifically made on the basis of an individual's genetic or cellular makeup are far more effective when it comes to ensuring a favourable treatment outcome or more effective management of disease conditions. In vivo microscopy is such a technique that will offer real time information to the doctors at the cellular level and would facilitate highly time-sensitive intervention and decision- making. In simple words, in vivo microscopy is a modern technique that involves the use of endoscopic tubes inserted into the human body for the purpose of obtaining images in real time. In vivo microscopy is definitely on its way in emerging as a revolutionary technology for patients suffering from critical conditions who require immediate diagnosis and treatment. Why in vivo microscopy: Traditional microscopic sample evaluation is a time consuming exercise involving sample collection, preservation, processing and examination in a lab and would normally require at least 24 hours before any diagnosis or treatment decisions are made. In vivo microscopy on the other hand will allow instant diagnosis of the condition or conditions without the need for removal of any tissue from the body. There is no doubt that in vivo microscopy will completely change the way health conditions are diagnosed and managed and will herald a change from the current biopsy and histopathological practices to a paradigm that will allow instant decision making and immediate intervention. Traditional microscopy that involves retrieval of tissues and elaborate histological procedures such as creation of tissues sections and staining is definitely very accurate and has been indispensable for medical diagnosis but it has limitations that can certainly impede rapid decision making and intervention.
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Histopathological procedures would requires carrying out biopsies that are stressful for patients and meaningful conclusions can only be drawn when the right tissue is retrieved. In essence, the process of sampling is critical and accurate diagnosis is only possible when the most relevant sections of the biopsy samples are analysed. Another issue with traditional histopathological examination is the possibility of contamination during sample preparation. The samples may undergo significant morphological alteration and thereby provide very limited information to the pathologist on the underlying medical condition of the patient. In vitro microscopy negates all the issues
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