8B-handout-mar2 - (you will see this in lab special...

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Chem 8B Structures of amino acids: (alphabetical order) H 2 N OH O H 2 N OH O CH 3 H 2 N OH O CH 3 H 3 C H 2 N OH O CH 3 H 3 C H 2 N OH O H 3 C CH 3 H 2 N OH O HO H 2 N OH O CH 3 HO H 2 N OH O HS H 2 N OH O S H 3 C H 2 N OH O O HO H 2 N OH O O H 2 N H 2 N OH O HO O H 2 N OH O H 2 N O H 2 N OH O H 2 N H 2 N OH O HN H 2 N NH H 2 N OH O H 2 N OH O HO N OH O H H 2 N OH O HN H 2 N OH O N NH glycine (Gly/G) alanine (Ala/A) valine (Val/V) leucine (Leu/L) isoleucine (Ile/I) serine (Ser/S) threonine (Thr/T) cysteine (Cys/C) methionine (Met/M) aspartic acid (Asp/D) glutamic acid (Glu/E) asparagine (Asn/N)) glutamine (Gln/Q) lysine (Lys/K) arginine (Arg/R) phenylalanine (Phe/F) tyrosine (Tyr/Y) proline (Pro/P) tryptophan (Trp/W) histidine (His/H)
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N H H N O R O N H R R H N O O R R H N O O N H HN O R O NH R R O HN N H O R O NH R R O Cu 2+ H N NH O R O N H R R O CuSO 4 10% NaOH peptide (2 or more amide bonds) peptide complex with Cu 2+ (red-violet color = positive test) (blue color) NH 2 R HO O amino acid building blocks peptide (amide bonds link amino acids) Biuret test: a colorimetic assay with Cu(II) ions to detect peptides and proteins
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Unformatted text preview: (you will see this in lab. ..) special reagents or enzymes Peptide Synthesis: (dehydration reaction removes water) NH 2 R HO O amino acid CuSO 4 10% NaOH (blue color) No peptide complex with copper No color chage (still blue color) N H H N O R O N H R R H N O O R R H N O O peptide (amide bonds link amino acids) Peptide hydrolysis: hydolysis in strong acid, strong base, or with enzymes H 2 O NH 2 R HO O amino acid building blocks (hydrolysis of amide bonds) (blue color = negative test) N H O R H 3 C H 2 O acid, strong base, or with enzymes simple amide hydrolysis: +...
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