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233hemoglobin_and_myoglobin_II

233hemoglobin_and_myoglobin_II - Chemistry 233 Fundamentals...

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Chemistry 233 Fundamentals of Biochemistry Hemoglobin and Myoglobin II
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Figure 10-6 Effect of pH on the O 2 -dissociation curve of Hb: the Bohr effect. The Bohr Effect This was observed by Christian Bohr and reported in 1904. Increasing acidity shifts the Hb binding curve to the right (away from the Y-axis )
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What physiological conditions affect the pH of the blood? M etabolism generates CO 2 and lactic acid CO 2 + H 2 O Æ HCO 3 - + H + lactic acid lactate HO CH C CH 3 OH O HO CH C CH 3 O- O H+ +
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What is the effect of the increasing acidity on Hb: Hb H + + O 2 Å Æ Hb O 2 + H + As metabolism increases, the pH goes down and more O 2 is released . Hb binds either protons or O 2 .
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Where do the “Bohr protons” bind? In the T -state of Hb, β C-terminal changes when O 2 binds:
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HN CH C O- O +HN N H NH CH C NH O C O- O HN CH C -O O N HN NH CH C NH O C O- O O 2 binding H+ T-state: Bohr proton R-state:
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Hb can also bind CO 2 at the N-termini : Carbamate formation increases the acidity of the blood and more O 2 released due to the Bohr Effect O C O protein H 3 N+ C NH protein O O- + H+ +
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Summary of the Bohr Effect:
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The BPG Effect bis -Phosphoglycerate (BPG) is a small triose found in the blood Note: 5 negative charges BPG binds to Hb in the central region between the subunits in the T -state
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Binding of BPG to the Hb tetramer BPG only binds to the T -state because the hole in the R -state is too small
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