Ruf 6 - Main Points Lecture 5 Trp operon trp repressor...

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Trp operon: trp repressor requires co-repressor (trp) for binding, regulation by transcriptional attenuation (controlled by availability of trp and stem-loop formation) 3:4 pair= attenuator = transcriptional termination Low trp= stalling 2:3 pair transcription of trp operon genes Main Points: Lecture 5
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Regulation of Gene Expression Intro and Overview, Prok. transcription Regulation of Prok. gene expression, regulatory proteins Concept of an operon: lac and trp Euk. Transcription, gene organization Regulation of Euk. gene expression Eukaryotic Gene Organization and Regulation
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Prokaryotic and Eukaryotic DNA Differ Greatly Information density: greater in prokaryotes than eukaryotes Association of genomes with protein: Prokaryotic genomes only loosely associated Eukaryotic genomes are tightly associated with histones and other chromosomal proteins: chromatin
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Differences in DNA for Prokaryotes and Eukaryotes E. coli 4.2 x 10 6 bases 6000 genes (700X) (5-6X) Man 3 x 10 9 bases 30-40,000 genes E.coli: 950 genes/Mb Man: 9.3 genes/Mb
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The packaging of genes on the chromosome is much less dense than in prokaryotes. The structure of the genes of “higher” eukaryotes tends to extend over large regions of the genome through the presence of intervening DNA sequence elements within the transcribed region: these are introns Introns are removed by splicing: a post-transcriptional process Splicing is partially the result of RNA acting as a catalytic agent, while accessory proteins and small nuclear RNAs greatly refine the process in complex eukaryotes.
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