Ruf 7 - Main Points Lecture 6 Gene density as a function of...

Info icon This preview shows pages 1–8. Sign up to view the full content.

View Full Document Right Arrow Icon
Main Points: Lecture 6 Gene density as a function of organism complexity 3 classes of DNA in eukaryotes: How was this determined? Chromatin: highly organized DNA-protein complex Nucleosome as the basic unit of organization: histone octamer + 146bp DNA Various stages of chromatin packing Euchromatin vs. heterochromatin
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Eukaryotic RNA Polymerase and the Transcription Control Sequences are Different From those in Prokaryotic Genomes Eukaryotic polymerases are multi-subunit complexes: 12-16 subunits Eukaryotic DNA polymerases do not directly bind to promoter containing DNA- ancillary factors perform this function There are 3 distinct RNA polymerase enzymes with distinct gene class specificities Pol I: rRNA Pol II: pre-mRNAs , some small non-coding RNAs Pol III: tRNAs, other small RNAs Originally defined by sensitivity to α-amanitin
Image of page 2
α -amanitin concentration tRNAs (+ others) 28s rRNA 18s rRNA 0 1 Low 2 Med. 3 High 4 total RNA Large Small Neg (-) Pos. (+) mRNAs Three Eukaryotic RNA polymerases: Classified by sensitivity to α -amanitin
Image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Type RNA class α -amanitin sensitivity RNA Pol I rRNA only Only very high levels RNA Pol II Pre mRNAs and a few small (non protein coding) RNAs Very low levels RNA Pol III tRNA and other small RNAs Intermediate levels Three Eukaryotic RNA Polymerases First Classified by α -aminitin sensitivity
Image of page 4
Minimal Pol II Promoter: has only 2 elements TATAA…….N 20 ……YYCAYYYYY…. N 24 …..AGAC Startpoint TATA Box InR DPE Initiator element Downstream Promoter Element TATA + InR: TATA Box containing core promoters InR + DPE: TATA-less core promoters
Image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 6
Sequential assembly of the general
Image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 8
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern