Ruf 8 - Main Points: Lecture 7 Assembly and role of GTFs...

Info iconThis preview shows pages 1–8. Sign up to view the full content.

View Full Document Right Arrow Icon
Main Points: Lecture 7 Assembly and role of GTFs What does a minimal eukaryotic pol II promoter look like? Phosphorylation of CTD of Pol II TBP as a universal factor Characteristics of Pol I and III promoters PPE and common txn factors
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 2
Transcription Factors are Bipartite, Modular Proteins:   They contain a DNA-binding domain and an “Activation  Domain” that interacts with components of the PIC Activators bind enhancer elements Repressors bind silencer elements
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Modular organization of Transcription Factors DNA binding domain: Zn fingers, Homeodomains, Helix-Turn-Helix Protein-Protein interaction domains: homo/hetero oligomers -usually dimers, though trimers are not uncommon. Basic helix-loop-helix, leucine zipper Regulatory Domain: Activation - acidic, Q-rich, P-rich, Ø-rich, RNA,… Repression -basic, HDAC binding peptides, Other Domains: not in all factors -nuclear localization, prointeraction domains, ligand binding, signal-responsive sites … Ch 28: 1087-1092 (previously assigned)
Background image of page 4
Enhancer Properties upstream or downstream of a gene or promoter Can work from within a gene Can work in either orientation : Can act positively (enhancer) or negatively (silencer) Location and orientation independent and function at a distance
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Enhancers are regulatory elements that act at a  distance by DNA looping  Enhancers:    act at a distance   orientation- independent   position- independent
Background image of page 6
Trans regulators of transcription Transcription regulatory proteins (transcription factors) Activators : proteins that, when bound to specific DNA sequences near or far away from the genes, activate transcription. Repressors
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 8
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 28

Ruf 8 - Main Points: Lecture 7 Assembly and role of GTFs...

This preview shows document pages 1 - 8. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online