Lecture 05 - Lecture 5 Mapping with Molecular Markers...

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Lecture 5 Mapping with Molecular Markers Mapping Functions and Probability Distributions Lod Scores
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Molecular Markers 1) Co-dominant 1) May be highly polymorphic 1) Neutral 1) Physically linked to DNA, allowing integration of physical and genetic maps 1) Can be found anywhere on chromosome Advantages:
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Types of Molecular Markers Commonly Used 1) R estriction F ragment L ength P olymorphisms “ Rif-lip s” 1) S imple S equence L ength P olymorphisms “ SSLP s” 1) S ingle N ucleotide P olymorphisms snip s”
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Restriction Endonucleases
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Non-specific Binding Specific Binding Linear Diffusion Coupling Catalysis Release RE DNA
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Southern Blot – used to detect presence and size of DNA fragment. Can you hybridize two double- stranded DNAs? Both Probe and Target DNA needs to be Denatured made single stranded. NaOH
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SSLPs/Minisatellite Repeats DNA Finger Printing – probe = semi-repetitive DNA, several polymorphic loci at one time
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TAGCGAGG CACACACACACACAC GTCGGGCTA ATCGCTCC GTGTGTGTGTGTGTG CAGCCCGAT TAGCGAGG CACACACACACACACACACAC GTCGGGCTA ATCGCTCC GTGTGTGTGTGTGTGTGTGTG CAGCCCGAT SSLPs/Microsatellite Repeats
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SSLPs/Microsatellite Repeats
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S ingle N ucleotide P olymorphisms Hybridization based detection with Molecular Beacons GGGACG T ACCTTAT GGGACG C ACCTTAT
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Alignment of Physical and Genetic Maps
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Distance B to M 37.6 B to P 32.2 P to M 26.9 Expected B to M 59.1 Alfred Sturtevant Columbia Fly Lab The linear arrangement of six sex-linked factors in Drosophila, as shown by association. 1913 Journal of experimental Zoology 14: 43-59. How Come Larger Genetic Distances are Generally
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Lecture 05 - Lecture 5 Mapping with Molecular Markers...

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