Lecture 06 - Lecture 6 Prokaryotic Genetics E coli Scale...

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Lecture 6: Prokaryotic Genetics
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E. coli
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1 μ M Yeast Bacillus Phage Scale
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Bacterial Morphology
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Gram Positive and Negative Bacteria Escherichia coli Staphylococcus epidermidis
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Bacillus subtillis : Gram Positive E. coli Model for cell differentiation and endospore formation
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Escherichia coli was first described by Theodor Eschrich, a German pediatrician and bacteriologist, in 1885 Present in the human gut. It is estimated that a human defecates between 100 billion and 10 trillion E. coli every day.
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Wild type E. coli can grow on minimal media Select mutants that are required for growth.
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Lac + = red, can grow on lactose Lac - = not red, can not grow on lactose
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Genome 4.6 MB Plasmids 1-200 KB, typically 3-10 KB Cell Division A plasmid is an extrachromosomal DNA molecule that is autonomously replicating, typically under copy number control, and usually circular and “supercoiled”. Plasmids have been heavily engineered for Molecular biology.
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Three ways to move DNA from one Bacterium into another 1) Conjugation: Bacterial sex 1) Transformation: Naked DNA uptake 1) Transduction: Phage hitchhiking
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1)Conjugation: Bacterial sex
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1)Conjugation: Bacterial sex Joshua Lederberg and Edward Tatum. 1946
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Conjugation Requires Contact
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1)Conjugation: Bacterial sex
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F plasmid is transferred as a single-stranded molecule.
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