Lab 5 Report - Thin Layer Chromatography The purpose of the...

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Thin Layer Chromatography The purpose of the experiment was to use chromatography to determine the identity of a given unknown analgesic. Thin layer chromatography involves a solid stationary phase, like a film of silica gel on a plastic sheet, and a liquid mobile phase. Four solvents were tested to find the most effective liquid mobile phase: 100% ethyl acetate, 92:8 ethyl acetate: acetic acid, 60:40 hexane: ethyl acetate, and 10:10:1 toluene: ethyl acetate: acetic acid. 100% ethyl acetate and 92:8 ethyl acetate: acetic acid were chosen based on the observation that the four painkillers were spread out across the TLC plates in a greater range. The good compound separation meant that the solvents had good polarity (not too polar nor not polar enough). After having chosen the solvents and assigning compounds, it could be observed that the unknown compound traveled in distinct conjunction with only one compound: acetaminophen. For both solvents, the unknown and acetaminophen traveled the same distance while aspirin traveled faster. In comparison to caffeine and ibuprofen, the
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