Lab 6 Report

Lab 6 Report - Identification of an Adulterated Herb or...

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Identification of an Adulterated Herb or Spice by Thin Layer Chromatography The purpose of the experiment is to identify the components of an adulterated herb or spice with the technique of thin layer chromatography. There were five TLC plates developed for each experimenter in the group, the first two of which were for known compounds that can be found in some herbs and spices to be used as a reference. The third TLC plate was prepared with the three pure samples given, and the fourth is cospotted with these along with the adulterated H/ S sample. The development of the fourth TLC plate revealed that the adulterated H/S sample, code number 812, iodine stains with two different R f values that were identical to that of the iodine stains of the pure H/S sample, code number 811. The R f values were .28 and .56. According to observations made from TLC plates 1 through 3, 811 contains 3,7-dimethyl,1-6-octadien-3-ol, which is also in 812. To find the adulterant, the development of the adulterated H/S sample was observed for
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Lab 6 Report - Identification of an Adulterated Herb or...

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