biologylab - Ana Laura Martinez-Tapia Nicole Botteri BSC...

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Ana Laura Martinez-Tapia Nicole Botteri March 28, 2007 BSC 2010-0502 Abstract Photosynthesis is a process used by plants, algae, some bacteria and some protists to form organic compounds driven by the reaction of light. The light reaction of photosynthesis is affected by two factors, the wavelength of light and the intensity of light . 2, 6-dichlorophenolindophenol ( DCPIP) will substitute NADP+ in accepting electrons in the process of photosynthesis. DCPIP is a blue dye in its oxidized state, and acts as an artificial electron acceptor. One can calculate the reaction rate of photosynthesis and how it is affected by the intensity and wavelength of light, and by observing the discoloration of DCPIP when measured by the spectrophotometer. The four wavelengths are 450 (blue light), 545 (green light), 650 (red light) and 750 (far red light). I hypothesize that optimal reaction rate for wavelength will be lowest at 545 nm because according to Campbell and Reece (2002), the 545 nm, represented by the green wavelength, will have no effect on the reaction rate of photosynthesis. I hypothesize if the intensity increases then the reaction rate of photosynthesis will increase as well. 2
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Ana Laura Martinez-Tapia Nicole Botteri March 28, 2007 BSC 2010-0502 Introduction Photoautotrophs, such as plants, algae, some bacteria, and some protists capture light energy to drive the synthesis of organic compounds, in a process called photosynthesis. Photosynthesis, driven by the presence of light, uses inorganic compounds carbon dioxide and water to make carbohydrate, water and oxygen. The following equation is found in Vliet Biology Lab Manuel to summarized photosynthesis: Light 6CO 2 + 12H 2 O => C 6 H 12 O 6 + 6H 2 O + 6O 2 Photosynthesis is comprised of two reactions, a light reaction and a dark reaction. Light reactions and dark reactions occur in the chloroplast of plants and algea, but more specifically, the light reactions occur in the thylakoids and the dark reactions occur in the stroma. A light reaction is dependent upon sunlight, which produces oxygen gas and converts ADP and NADP+ into the energy carriers ATP and NADPH. A dark reaction is
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biologylab - Ana Laura Martinez-Tapia Nicole Botteri BSC...

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