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L15 chapter 14

L15 chapter 14 - Genetics Biology 2306.04 Ch 14 RNA...

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Unformatted text preview: Genetics Biology 2306.04 Ch 14: RNA Molecules and RNA Processing Not true in eukaryotes Coding sequences (exons) in a gene may be interrupted by noncoding sequences (introns) Introns rare in bacteria; common in eukaryotes mRNA Structure pre-mRNA Processing Eukaryotes (occurs in nucleus) addition of 5’ cap addition of 3’ poly(A) tail removal of introns Addition of 5’ Cap Modified nucleotide • usually 7-methyl guanosine • 5’-5’ bond • methylation of additional nucleotides addition of 5’ cap Function of 5’ Cap binding of ribosome to mRNA for translation stabilizes mRNA affects intron removal Addition of the Poly(A) Tail Poly(A) tail • 50-250 A nucleotides at 3’ end of mRNA Two steps • cleavage of 3’ end • polyadenylation addition of 3’ poly(A) tail Function of poly(A) tail Increases stability of mRNA Required for ribosome binding to 5’ cap Intron Removal (splicing) Occurs in the spliceosome • snRNAs (U1, U2, U4, U5, U6) + proteins • snRNPs = 1 snRNA + proteins Requires consensus sequences pre-mRNA Alternative RNA Processing...
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L15 chapter 14 - Genetics Biology 2306.04 Ch 14 RNA...

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