Ch6 - Analysis of unlabeled proteins by Western blot...

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Content List for Chapter 6 Affinity k 1 : forward or association rate constant k -1 : reverse or dissociation rate constant K a : equilibrium constant for association or association constant K d : equilibrium constant for dissociation or dissociation constant Equilibrium dialysis Scatchard equation o Scatchard plots o Slope=-K a o X-axis intercept = n = valence of Ab o Linear vs. curved lines Avidity IgM: avidity compensates for low affinity Cooperativity Cross-reactivity ABO blood types Precipitin Reactions Bivalence or polyvalence of Ab and Ag Equivalence zone Immunoprecipitation Collection of the Ag-Ab complex: secondary Ab, precipitin, protein A, magnetic beads Analysis of labeled proteins by polyacrylamide gel electrophoresis and autoradiography
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Unformatted text preview: Analysis of unlabeled proteins by Western blot Western blot Polyacrylamide gel electrophoresis SDS Membrane transfer Detection with radiolabeled or enzyme-conjugated Ab Agglutination Agglutinins Prozone effect Incomplete antibodies Passive agglutination Agglutination inhibition Radioimmunoassay (RIA) Competitive binding ELISA (Enzyme-linked immunosorbent assay) Indirect ELISA Sandwich ELISA Competitive ELISA ELISPOT assay Immunofluorescence Fluorochromes Microscopy Fluorescence-activated cell sorting (FACS) Direct vs. Indirect detection Secondary or indirect detection reagents...
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This note was uploaded on 04/28/2008 for the course MMG 451 taught by Professor Brooks during the Spring '08 term at Michigan State University.

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