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Unformatted text preview: Analysis of unlabeled proteins by Western blot Western blot Polyacrylamide gel electrophoresis SDS Membrane transfer Detection with radiolabeled or enzyme-conjugated Ab Agglutination Agglutinins Prozone effect Incomplete antibodies Passive agglutination Agglutination inhibition Radioimmunoassay (RIA) Competitive binding ELISA (Enzyme-linked immunosorbent assay) Indirect ELISA Sandwich ELISA Competitive ELISA ELISPOT assay Immunofluorescence Fluorochromes Microscopy Fluorescence-activated cell sorting (FACS) Direct vs. Indirect detection Secondary or indirect detection reagents...
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This note was uploaded on 04/28/2008 for the course MMG 451 taught by Professor Brooks during the Spring '08 term at Michigan State University.
- Spring '08