{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Ch6 - • Analysis of unlabeled proteins by Western blot...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Content List for Chapter 6 Affinity k 1 : forward or association rate constant k -1 : reverse or dissociation rate constant K a : equilibrium constant for association or association constant K d : equilibrium constant for dissociation or dissociation constant Equilibrium dialysis Scatchard equation o Scatchard plots o Slope=-K a o X-axis intercept = n = valence of Ab o Linear vs. curved lines Avidity IgM: avidity compensates for low affinity Cooperativity Cross-reactivity ABO blood types Precipitin Reactions Bivalence or polyvalence of Ab and Ag Equivalence zone Immunoprecipitation Collection of the Ag-Ab complex: secondary Ab, precipitin, protein A, magnetic beads Analysis of labeled proteins by polyacrylamide gel electrophoresis and autoradiography
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: • Analysis of unlabeled proteins by Western blot Western blot • Polyacrylamide gel electrophoresis • SDS • Membrane transfer • Detection with radiolabeled or enzyme-conjugated Ab Agglutination • Agglutinins • Prozone effect • Incomplete antibodies • Passive agglutination • Agglutination inhibition Radioimmunoassay (RIA) • Competitive binding ELISA (Enzyme-linked immunosorbent assay) • Indirect ELISA • Sandwich ELISA • Competitive ELISA • ELISPOT assay Immunofluorescence • Fluorochromes • Microscopy • Fluorescence-activated cell sorting (FACS) Direct vs. Indirect detection • Secondary or indirect detection reagents...
View Full Document

{[ snackBarMessage ]}

Ask a homework question - tutors are online