Molecular Biology

Molecular Biology - October 10, 2006 Biology 101 Lab...

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October 10, 2006 Biology 101 Lab Molecular Biology Polymerase chain reaction, PCR, is a molecular biology technique which takes a small amount of template, and copies it exponentially. This technique has allowed scientists to spot mutations, use DNA to identify criminal suspects, as well as sequence the human genome. DNA samples that were too minute to work with can now be amplified enough to be experimented on. The template used in this lab was our own DNA, extracted from our cheek cells. We will use PCR to make copies of the PV92 locus of chromosome 16, which has two different forms in humans; one contains an extra repetitive sequence that increases its length. However, whether or not your DNA includes this Alu element isn’t an indicator of disease or any other known flaw. The purpose of this experiment was to determine if our DNA contained this extra Alu element, and whether our genotype was homozygous positive, heterozygous, or homozygous negative. Another purpose was to learn the importance and various applications of PCR, and gel electrophoresis. At the commencement of the experiment, each student began with one cup containing 10mL of 0.9% saline solution, one screw cap tube containing 200µL of InstaGene™ matrix, one empty 1.5mL micro test tube, and one empty PCR tube. First, we labeled all of the tubes and cups that belonged to us, so as not to mix samples. Next we rinsed the saline solution vigorously in our mouths for 30 seconds, and dispensed the saline solution, now containing some cheek cells, back into the cup. From the cup, we poured 1mL of the cheek cell/saline solution into the 1.5mL micro test tube
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we were all equipped with. Then the class placed their micro test tubes into the centrifuge, and allowed it to spin at maximum speed for 2 minutes. After the centrifuge stopped spinning, all of our micro test tubes had a small,
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Molecular Biology - October 10, 2006 Biology 101 Lab...

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