Lab #3 - Separation of Food Dyes and Separation of Lycopene...

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Separation of Food Dyes and Separation of Lycopene Sylwester Czado Chemistry 191 Lab Report, Experiment #3 Lab partners: Ellen Richer, Michael Folcarelli Brian Corbett 9-29-05
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Introduction The purpose of doing this particular experiment was to be familiar with chromatographic separation techniques by using paper chromatography and thin layer chromatography. This is done to separate mixtures of food color dyes and plant pigments. We are using this particular experiment because it is difficult to separate substances with similar physical and chemical properties. Separation of a mixture of closely related substances is a problem. That is why we use the powerful separation techniques called chromatography. Procedure The supplies that are needed for Separation of Food Dyes are: four colors of food dyes (blue, green, red, and yellow), Whatman No.1 filter paper, 46 x 57 cm sheets each pre-cut into twelve 11.5 x 19 cm pieces, centimeter ruler, pencils, applicators, plastic food wrap, size 33 rubber bands, scissors, and a stapler. The supplies that are needed for Separation of Plant Pigments are: canned tomato paste, 15 x 125 mm and 13 x 100 mm test tubes, test-tube rack, transfer pipets, spatula, hot plate, centrifuge, #3 and #4 size corks, plastic food wrap, 10-mL beakers, 2.5 x 7.5 cm J.T. Baker’s Baker-flex Precoated Flexible TLC sheets, Silica Gel IB-F coating, developing chamber (4-oz wide-mouth screw cap bottles with Teflon-lined screw caps), applicators, and pencils.
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Safety Precautions: Petroleum ether, acetone, and toluene are all flammable solvents. There must be no open flames in the laboratory while the experiment is performed. To minimize exposure to the vapors of the solvents, work in a fume hood. Waste Collection: The solvents used for the separation of plant pigments should be collected in a bottle labeled “Waste petroleum ether, acetone, and toluene.” 1. Cut an 11.5 x 19 cm piece of Whatman No.1 filter paper. Draw a line parallel to the long dimension about 2 cm from the edge with a pencil and not a pen. Put four small Xs on the line beginning 5 cm from the edge of the paper
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Lab #3 - Separation of Food Dyes and Separation of Lycopene...

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