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18.FA08 - NAD H 2 O How is electron transport coupled to...

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Metabolic Biochemistry BIBC102 Lecture 18 November 5, 2008
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Free Energy” + 2 + 2
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mistake in text book
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An excerpt from Table 13-7, in reverse order But note there is a mistake in Table 13.7 for the reduction of ubiquinone pH = 7
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An excerpt from Table 13-7, in reverse order But note there is a mistake in Table 13.7 for the reduction of ubiquinone pH = 7
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2 NADH + 2 H + + O 2 ==> 2 NAD + + 2 H 2 O the half reactions: NAD + + H + + 2e - <===> NADH E o ' = - 0.315 V 1/2 O 2 + 2 H + + 2e - <===> H 2 O E o ' = + 0.815 V NADH + 1/2O 2 + H + <===> H 2 O + NAD + E o ' = 0.815 + 0.315 = 1.130 V [ E o ' = E o ' (electron acceptor) - E o ' (electron donor) ] G o ' = - n F E o ' where F is the Faraday constant = 96,494 kJ/volt . mole, and substitution yields G o ' = - 2 x 96,494 x 1.13 = - 218 kJ/mole (NADH) since it takes 30.5 kJ/mole ATP to make ATP from ADP and Pi, we can in principle make 218/30 = ~7 moles NADH + H + + 1/2O 2 ==>
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Unformatted text preview: NAD + + H 2 O How is electron transport coupled to ATP synthesis ? LNC Fig.19.15 4H+ 2H+ 4H+ IMS MATRIX Proton pumping and Storage of Free Energy + + + + + +------Matrix IMS inner membrane LNC 19-6 ∆ G = 2.3 RT ∆ pH + 1 x F x ∆ Ψ ∆ pH = ~ 0.75 ∆ Ψ = ~ 0.15 - 0.2 v ∆ G = ~ +20 kJ/mol (H ) The oxidation of NADH liberates ~ 220 kJ/mol (NADH) therefore, we can pump ~ 11 protons at 100% efficiency tightly coupled vs uncoupled mitochondria succinate LNC 19-18a P. Mitchells’ Chemiosmotic Hypothesis Effect of antibiotics valinomycin and nigericin Valinomycin is a K + ionophore Nigericin is a H + /K + antiporter it exchanges protons for potassium ions and thus ATP Synthase Complex V F o F 1 ATP SYNTHASE F 1 : α 3 β 3 γ δ ε F : a b c End of Lecture 18 November 5, 2008...
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18.FA08 - NAD H 2 O How is electron transport coupled to...

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