19.FA08 - o turn 3 ATP / 9 PROTONS 3 ATP per OXYGEN (or per...

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Metabolic Biochemistry BIBC102 Lecture 19 November 7, 2008
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BIBC102.FA08 Midterm 0 5 10 15 20 25 30 35 40 normalized grade No. of Students 50 60 70 80 90 100 %
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ATP Synthase Complex V
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F o F 1 ATP SYNTHASE F 1 : α 3 β 3 γ δ ε F : a b c
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The ATP/ADP binding sites are located at the interface of the β and α subunits
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The engine can turn in both directions: with ATP hydrolysis it turns in the opposite direction when compared with ATP synthesis driven by proton flux into the matrix
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Calculated rates from v give rates of ~ 100-150 rpm
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ATPsynthase Home page for John Walker’s group http://www.mrc-dunn.cam.ac.uk/researc
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Interesting questions: How many protons enter per ATP produced (per 120 o turn)? How many c-subunits per γ subunit?
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A simple estimate 10 PROTONS pumped per NADH oxidized 10 PROTONS pumped per OXYGEN consumed 3 PROTONS pass through the ATP synthase per 120 o turn 1 ATP is made per 120 o turn Therefore: 3ATP per 360
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Unformatted text preview: o turn 3 ATP / 9 PROTONS 3 ATP per OXYGEN (or per NADH oxidized): P/O ratio = 3 In intact, well coupled mitochondria oxygen consumption depends on a supply of ADP and Pi In intact, well coupled mitochondria oxygen consumption depends on a supply of ADP and Pi succinate Experimental measurements of P/O ratios: P/O = ~ 2.5 for NADH P/O = ~ 1.5 for succinate Is that a problem? NO! There are other ways for protons to go back to the matrix without passing through the ATPsynthase: COUPLING is not perfect Total quantity of ATP produced per glucose oxidized to CO 2 (under aerobic conditions) Assignment for the Sections next week To be discussed in a future lecture for NADH: P/O ratio ~ 2.5 for succinate: P/O ratio ~ 1.5 Assignment for the Sections next week End of Lecture 19 November 7, 2008...
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This note was uploaded on 03/21/2009 for the course BIBC 100 taught by Professor Nehring during the Fall '07 term at UCSD.

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19.FA08 - o turn 3 ATP / 9 PROTONS 3 ATP per OXYGEN (or per...

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