Exam1andKey2008 - Page 1"810 digits/initial GENE/BIOL3200...

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Page 1 "810" digits/initial GENE/BIOL3200 Exam II Spring 2008 100 points total Enter the last 5 digits of your "810" number and the initial of your last name on each page. IF YOU DO NOT KNOW YOUR 810 NUMBER THEN LOOK IT UP ON YOUR CARD OR GIVE US A FIRST INITIA L AND LAST NAME . There are 14 questions as before, 6 worth 10 points each and 8 worth 5 points each. Good luck and good thinking. Remember, accuracy coupled to brevity is the soul of an A. There are 2 bonus questions worth 5 points each at the end of the exam. Deal is, you gotta’ get at least 4 points to get credit. Exams and scores will be available in Genetics Office, Life Sciences, next Tuesday. WE STILL HAVE FOUR UNCLAIMED WHITE EXAMS...!
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Page 2 "810" digits/initial 1. (5 pts) Six r mutants of bacteriophage T4 ( a. b, c, d, e, & f ) were each crossed to each other with the following results. Crossing mutants a, c, d, and f to each other gave rise to wild-type recombinants. Mutant b gave rise to wild-type recombinants with a and f , but not with c and d while mutant e gave rise to wild-type recombinants with c and d . When mutants b and e were crossed to each other, wild-type recombinants arose. What is the nature of the b and e mutations? Nonoverlapping deletions (b spanning c and d mutants while e spans a and f mutants). 2. (5 pts) Explain how a eukaryotic insulator functions. Why is this an important feature of eukaryotic gene regulation? Use diagrams if you want. A insulator acts to prevent enhancers for one gene acting on neighboring genes. This is important because enhancers can act over very long distances and also allows for their gene specificity (+1 bonus). 3. (10 pts) In table A below can be found recombinant frequencies of 4 mutations in the rII gene of T4 as observed in pair-wise crosses. Assemble the mutations into a genetic map with the distances between them. A "+" indicates that they complement each other and a "-" indicates that they do not complement each other. Map the genes and give the distances separating them.
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