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WI09MT28amtestandkey

WI09MT28amtestandkey - BICDIOO WWTER 2007 MIDTERMZ QUESTION...

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Unformatted text preview: BICDIOO WWTER 2007 MIDTERMZ QUESTION 1. 5 POINTS. TOTAL EARNED. You ar'“e studying three genes in film flies whose recessive mutant alleles have the r" phenotypes of peach-colored eyes (peach), .fine—veined wings (flashes), and antennae tremor (tremor). All three genes areknown to be X-linked. You cross female fruit flies from a pure—breeding tremor strain to male fruit flies from a pure breeding peach fine! ine strain to obtain F1 flies. You cross the F1 females to Wild—type male flies and analyze 2000 F2 male progeny. You obtain the following data: inferred gamete alleles mutant uheno -e(s) meltne each n‘emar _— 141 peach + .4. 104 I wild type ‘ l E + I 721 tremor + .Jf. —t I : ll fineline l E + + \ 707 fineline peach , _ 4- 102 fineline peach tremor - e t fineline tfemor P + t “.13 + + “he. a. State the genotype of the F1 flies, both male and female. m lamina get/M: {747’} t + -r 07' t++ met, Jewel. mtg - ' v '- egtumnt emumnt {smut-«1+ ‘ 1-1-9 (2+1 1" ? ? Y .k k F. ll 1-,; 1 "\Foab. Fill in the inferred gamete genotypes in the table above (the gamete produced by the F1 female fly inherited by the indicated F2 male fly phenotypic class). 3A Page 3 of 10 BICDl 00 WINTER 2007 MDTERMZ c.‘ From these data, derive the most accurate map you Can of the order of these genes and the distances between them. Draw your map. Vite ‘1" I +?§- t??? xv,“ QFT-Z‘ HOLHFLSHOlmoocfls “4th P’s?" p+ +5..- 2,030 i ’ Rf"- Zt+tLll+25rfl°t I-IOO le ‘ . ql 2:000 C 33M I Hometown—Fl ‘-'—'—-'—'——-—— fifC'k' : u Loco c M 26 ”’xM r2 IELM IE7: OM 43::._____ti————' ‘6' P 4:- _’,.____t ‘ 2575M log-e6. H {212: )(f\ d. Is there interference in this chromosomal region? Show calculations to support your answer. #ecpededDCO= (32.6% tr‘jtt'zpos‘t: (46.1% 5(\ eomves‘Dm: ewe-13% reserves/ewes. 000 The Observer) BED stem W straw/{two 11m, erg-meet OCOJhksmgeéeEEm ‘3 W was“ new. athbwmeéWerowwm i”: not: ‘5' Wham HM“! W SW3“ WM" WAGMH-‘é-W #‘ZbFJCM is, 993:. evidmtgiw m-terkrema. Even wfi’fi ouc werQQrmuso do Mapgcpm mg distort-ea 4% 0‘56 ‘1? bQOQUEE’.» OJ;- H19- ECO Page4 oflo Le {U BICDIOO WINTER 2007 MIDTERMZ QUESTION 2. 5 POINTS. TOTAL EARNED: in a yeast species that produces unordered tetrads. you are studying three genes. A (on chromosome 1) and B and C (both on chromosome 2). The B gene is closer to the oentromere than the C gene. You cross an A B C haploid with an a b c haploid to obtain a diploid. a) Diagram a meiosis of the resulting diploid with a single crossover between B and C. The diagram should clearly indicate which ohromatids segregate together in order to explain the alleles present in all four spores. for all three genes. _ "fiftiymassify the resulting tetrad as a PD, NF'D, or ii": P332 59$. AB FbC PIE) .2. cub b C MID be Page 5 of 10 BICD 1 00 WINTER 2007 MIDTERMZ o) What kind(s) of crossovers (XO's) in meiosis could generate a Tetratype ‘ (1T) tetrad for the A and B genes? (check all that apply) E] No XO's |:] Single X0 between A and B |:| Single X0 between A and C E] Single X0 between B and C Single X0 between A and its centromere \l Single X0 between B and its centromere [I Single X0 between C and its centromere |:| Double X0 between A and B 1:! Double X0 between A and C A B C |:] Double X0 between B and O /-"”f -- .04- _..._—r' A 3 C; > A /' //’_"—L\ -§——4- b: — %:L- - —- e E: L ___; o N A e—‘V’J" Page 6 of 10 C5: BICDIOO WINTER 2007 MIDTERM2 QUESTION 3. 6 POINTS. TOTAL EARNED: In yeast Sacchammyces cerew'siae you cross two haplolds: a met2 x a argT. Remember that a and a are alleles of the MAT gene, so there are three genes segregating in this cross. From the following unordered tetrads. determine which genes are linked, and calculate the distance between any linked genes. I II 111 IV a ma! (tr-5+ a men-l- arg+ :1 mm arti- a mm- ar3+ a ma: air-3+ a mer+ cry a mu: erg? a mar:- erg? a mr+ org? a ma: erg? umm- arg+ am: org+ Ma r ? M M er+ '7 m1 119 125 33 27 Oxmeio'l + x- oire m5? ‘lr Oxmekl‘l' at + my WTWGV-LRD 1mm 11.?0 1»:me ©90=nVDmunhed @nfl":. loo't’hqiam ®_ TTF'WW? ("to n?!) glssinaie mu d‘fitaflfle. MAT—H25: lift-TL I 00‘ M ml '=f.,_~l3:3+11') fit “in a n9 +3927 1:. qu 37 EM “FT ILTTQ ?D=nPD 2 a mm W TT?(‘€5€.D+ j, MOS‘Qef‘Lb b Yb’f" Meme immam “Wm meta .. 3111(2 . - .. - 4-— ummnm kflgithfi (mam. b bfi’i‘hm of)?“ M WT} ”l \‘f “MT—£6) Emir cent-rem L 1‘33“. dnd'bt- eebmiame \‘in\'—ed+oilrs Gem“ l k5) $3.15an WU gr . Mo) L [T13 .L 6:)“: cm “‘5 cenm: M‘T 2' m “WM?- 1 fillinmz‘tfi’lgr Page 7 of 10 5°“ BICDIOO WINTER 2007 MIDTERMZ ' QUESTION 4. Ali-POINTS. TOTAL EARNED; Normal bacteriophage can grow at either high or low temperature. You have many strains of bacteriophage that are temperature sensitive (unable to grow at high temperatures). You co-infect two of these temperaturesensitive strains of phage, m1 and m2. into bacteria at low (permissive) temperature. and then when the cells lyse you plate out progeny phage on a new bacterial lawn at high (restrictive) temperature. 22 plaques grew on the high temperature plates, out of 4000 phage particles plated. rtt a) The 22 pla-ues arise by which mechanism: complementation. interference, or nondis'unction? (circle unanimous) (\rebmgisenbih‘ qun mm (admiss- WNW W W “- Peeiwrtiw cram obwwe ‘3“er W5 W mutant) weaker crew, (WWW. l-o wWimel fighter“? but it 'm'iw)‘ b) Calculate the map distance between m1 and m2” “5" WWVW-q QUE WEM (3ng be *5 W3 i. mt ' [‘1‘ “11 l X \ fl '2 whee . 4-, -1—i-' ] Pig—llZl-thmar-AZ‘JQM m1 Jr + “(35‘1” (4,000 / é ex , Moimm Mi i” Mi *t Page 8 of 10 BICDIOD WINTER 2007 MIDTERMZ QUESTION 5. 4 POINTS. TOTAL EARNED: You have a wild-type strain of E. coli with the genotype A B C D E F G You introduce an F+ plasmid into your. wild-type strain and isolated two Hfr derivative strains that you call Hfrl and Hfr2. You are studying several new genes in E. coli with interesting phenotypes. You obtain a multiply mutant strain with chromosomal genotype: abodefg You mate each Hfr strain to your multiply-mutant strain in a separate experiment. At various times you interrupt the matings and. plate the bacteria under conditions in which only the recipient strain can grow. The chart below shows the earliest-«time-of-entry for each gene, in minutes: “— Draw a map of these genes that is consistent with the data, including all the genes and Hf: origins, the distances between them (in minutes), and the direction of transfer of each Page 9 oflO ...
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