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WI09MT2testandkey

WI09MT2testandkey - BICDIOO WINTER 2007 MIDTERM2 ll” M w...

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Unformatted text preview: BICDIOO WINTER 2007 MIDTERM2 ll” M w V5 QUESTION 1. 6 POINTS. TOTAL EARNED: ' Y on are studying three genes in fruit flies whose recessivelmutant alleles have the 4“”), q 41 phenotypes of peach-colored eyes (peach), fine-veined wings (fineline), and antennae ML ._ F tremor (tremor). All three genes are known to be éefinked. You cross female fruit flies from a gore-breedingtremg; strain to male fruit flies from a pure breeding peach fineline fit. -.‘A ‘— f strain to obtain F1 flies. You cross the F1 females to wild~type male flies and analyze 2000 F2 male progeny. You obtain the following data: inferred gamete alleles . I‘ # mutant nheno pB(S) iflr L? a. State the genotype of the F1 flies. both male and female. 1‘? " "I 4 I +r + F lvi..j....__ \ "LKI‘F’ f (M!) 1‘, P4 5" 5: J“ 13. Fill in the inferred gamete genotypes in the table above (the gamete produced by the F1 female fly inherited by the indicated F2 male fly phenotypic class). 1!: gala. Whlwl 10" 3 4*)? MP7! f [3* z p? “(a-u #7:: 3' ffl‘sn‘? (Jfirfif I {Y ‘i 4Y":&'H"'f I Page 3 of 10 BICDIOO WINTER 2007 MHDTERMZ c". From these data, derive the most accurate map you can of the order of these genes and the distances between them. Draw your map; 1: m w, L. View. 2w anew M“- Ma " ’4 r 4"? ‘4‘ F P‘H’Hg‘l CI - 4M1.” 10 I?" z: =69 M + r 3”»: ‘ ..aHJ fi-‘FT-flF—v—fi - z: 3;}... 44 F " ”Ml M 1 = /5'.? M4 E IEH43};.__a-,(—————W.'iv—3 N ,er —+———4——————~—+— 'a __£--—-—' f P .F ”41' 1“ '44 2g.¢r;.’ wfpzam of I} .-- CJ, 1 '14,;o I)“ _ __ __.. _'_‘_ -. ...._ .. I ‘4". h .Lr' .. J “ , WW 3w»- .. . - a“ _r 4 = r r . .. Hf" 4 4, n3 I83. cM -. #114 5.1"" d J' 'r' _..__. fl... — -— y and F kr-l E #119. u (I! ’l .. d a .a- a F '* - 4 r57; foi‘f' 4 4-! "- ’91 F?“ F fa", 2’ I; +{ :' ”If { HT 1-. g" 2 r fi-‘l d. Is there interference in this cfirfi’rfi'firfimal region? Show calculations to support your answer. OLWJ‘. Clare-412 kt? : H“? (E '5' 2" +\ 6’24“”; 'J’“‘*":' 3"" ‘ (eureflwfrrhowfi = 51,925 chased, 333W Lw4wu- X“ "if: T /-- Page 4 of 10 BICD 100 WINTER 200'? MJDTERM2 QUESTION 2. 5 POINTS. TOTAL EARNED: in a yeast species that produces unordered tetrads, you are studying three lit? (3.. g genes, A (on chromosome 1) and El and 0 (both on chromosome 2). The B gene (A i" is closer to the centromere than the C gene. You cross an A B C haploid with an a b c haploid to obtain a diploid. a) Diagram a meiosis of the resulting diploid with a ’ la it between Wage. The diagram should clearly indicate which chro‘fififids segregate together in order to explain the alleles present in all four spores, for all three genes. b) Classify the resulting tetrad as a PD. NPD, or 'l_‘_l'__for each gene pair. I! T A + B C \_‘\“ a) 'r A is c 55¢, EH ‘ ' it": E?- .‘—"""3' fey.“ °~ ‘- ' ' i a. J'__§_...§_ _ A??? J, g-‘j-T '. FE; firth—'5' m C “flit-Kurt»... A- s ‘i T rs- C W A ,L. TT + l Page 5 of 10 BICDIOO WINTER 2007 MIDTERMZ o) What kind(e) of crossovers (XO's) in meiosis could generate a Tetretype ‘ (TT) tetrad for the B and 0 genes? (check all that apply) [:1 No XO's "“"’“” W“ II] Single X0 between A and B II] Single X0 between A and C I Single X0 between B and C + K [:1 Single X0 between A and its centromere D Single X0 between B and its centromere D Single X0 between C and its centromere [:| Double x0 between A and B Double X0 between A and 0 Double X0 between B and C so“ a” w eec : 9: c. ‘Zm. f e l c m;— E“: _fl 7—,.— E‘a '5' -—--:'i \QC ’ 1 1 e C ———-e w c. g r frfl? M 1“?”“7 E: rec ” 5C, l he EL 3 En EC. Page 6 of 10 BICDIOO QUESTION 3. 6 POINTS. ln yeast Sacoharomyces cerevisiae WINTER 2007 MIDTERMZ TOTAL EARNED: you cross two haploids a trpa x o: hls2. Remember that segregating in genes are Ilnk _________,____ I K III IV a am a 3m.” afl'pJMH- appaltu+ («can +944! 6 1092? 3333134- ag3fis+ cup-1.135.}. 3mm ew— p aflP+M12 amen). a JMH awn“: a V916 + M M E + " m ’1 '5 10 16 / t 7%? fl“ — in I“ Mfi'r ‘- Alt ,Lfli— m 1 fl? Pl? Pp I “(’9 l (W? PD i1. 77 NW Tr W 71 PP 'TT / .—~—' WWW Mfr .. .__.._..HJII --"'""'""" Pp», ”so ,5: WEE-i.) M p 5: ~FO¢NP§ 5' ' It?“ ‘— 9L1: “:th wow l .. (9-; NEW , 9 a N’ 4““‘J" ‘ [IV ' ‘ v i {Tr‘l l: l I’ .. A22 77*_ 5" _ ”41"“ .__ 1..—-—-—l v ‘0- .-- 9rd— ol . [J {I J ft, 4' If? {gut-5Q! a fit... (2.1?" tic-v"? "‘4‘ W51 7t .- _L(-fb't Ia Jtfiwttrr‘ 41‘ ‘1‘" H ’l Ltd-7w '- L—r—hv a: hf. In} 1,9? {V {fffkm-r I? 1"3“J'"-" (not; w W" . M «(a if ktufi 1190 1‘ Qifiifl— wuitflrdflkw 7 .Lmt " 391 I}? [LI‘ . —— H____._¢----—‘ __ .. |[ / tr I P . F. 5 MM 9L k L 1:: fife M [Ma La. __'__"f_—/’/ e and on are alleles of the MAT gene. so there are three genes this cross. From the following unordered tetrads. determine whlch ed. and cajgglatgthe diatanoehmy linked gmenes. Page 7 of 10 [)9 M w BICD 100 WINTER 2007 MIDTERM2 5 QUESTION 4. 4! POINTS. TOTAL EARNED: Normal bacteriophage can grow at either high or low temperature. You have many strains of bacteriophage that are temperature sensitive ( unable to grow at high temperatures). You co-infeot two of these temperature—sensitive strains of phage, m1 and m2. into bacteria at low (permissive) temperature, and then when (restrictive) temperature. 57 plaques grew on the high temperature plates. cut of 5000 phage particles plated. I a) The 57 plaques arise by which mechanism: interference. M .....'.-—---—-——~l-~ u _M‘-L:_:'“—"E:“—- 1...:- 27 A" firm plot-299d ._.-n - \E l 1' . _ ‘ L Fl 0'4"“ 3”“! I“ Fig.2.”; b) Calculate the map distance between m1 and m2 fl r€L?mb-Ilfi’~;:-I 1 S?- FF:"-: I w... I .. ., gum a g rrflmhllk‘flh A ii ,. flow In —' - ‘- H. fl F a" F I Page 8 of 10 complementationiuracembinationgor nondisiofiction? (circle one) BICDIOO WINTER 2007 .MIDTERM2 QUESTION 5. 4 POINTS. TOTAL EARNED: You have a wild-type strain of E. coli with the genotype ABCDEFG You introduce an F+ plasmid into your wild-type strain and isolated two Hf;- derivative strains that you call Hfrl and Hfr2. You are studying several new genes in E. coli with interesting phenotypes. You obtain a multiply mutant strain with chromosomal genotype: abcdefg Draw a map of these genes that is consistent with the data. including all the genes and Hfr origins, the distances between them (in minutes), and the direction of transfer of each Hfr. Fri A H \l\ Page 9 of 10 \)\\ ...
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